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Experiment in midbrain primary cultures, enriched in dopaminergic neurons

ABSTRACT: To identify novel Nurr1 target genes we have used microarrays strategies in rat midbrain primary cultures, enriched in dopaminergic neurons, by the action of basic fibroblast growth factor (bFGF, 20ng/ml) and Sonic hedgedog (SHH), following upregulation of Nurr1 expression by depolarization.To this aim we have treated the cultures after 9 days in vitro for 2h with high KCl and collected 30 min or 2 h after the end of depolarization (2h + 30 min or 2h + 2h). With this experimental protocol we have identify a putative Nurr1 regulator and Nurr1 target Keywords: depolarization, microarrays, time course.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE4551 | GEO | 2007/03/19

SECONDARY ACCESSION(S): PRJNA94469

REPOSITORIES: GEO

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