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Gene expression profile changes during blastocysts hatching of embryos produced from parthenogenesis and in vivo

ABSTRACT: Comparative transcriptomic analyses among in vivo (IVV) XB, in vivo (IVV) HB, parthenogenetic activation (PA) XB, and parthenogenetic activation (PA) HB were performed following a reference design.

ORGANISM(S): Sus scrofa

PROVIDER: GSE48290 | GEO | 2016/06/19

SECONDARY ACCESSION(S): PRJNA209524

REPOSITORIES: GEO

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Comparison of hatched blastocyst derived from IVV, PA,and CT

Project description:Comparative transcriptomic analyses among in vivo (IVV) HB, parthenogenetic activation (PA) HB, and chromatin transfer (CT) HB were performed following a reference design.

2016-06-19 | GSE48291 | GEO

Analysis of gene expression in uniparental mouse blastocysts produced in different culture media

Project description:In the field of in vitro embryo production it is generally thought that the culture media are not as good as the oviductal fluid, resulting in detrimental changes of embryonic gene expression. Yet in vitro embryo culture (IVC) is one of the pillars of the assisted reproductive technologies. Therefore, unintended effects on gene expression may impact on the health of ART babies (PMID 27554442). Granted, human ART has many more components than just the culture media, but, the practice of embryo culture superimposes with the phase of life when cellular totipotency is present - that's why embryo culture media receive special attention. Discerning what culture media do is difficult because several confounders are present, including but not limited to oocyte heterogeneity, sperm heterogeneity as well as time of fertilization. We propose to take advantage of experimentally produced parthenogenetic embryos, and thereby remove two confounders (sperm variability, time of fertilization), in order to assess the effects of specific culture media of embryonic gene expression, in the mouse model of human reproduction. After synchronous parthenogenetic activation of MII oocytes, pronuclear oocytes were cultured in parallel in ART medium vs KSOM(aa) medium. Resultant blastocysts were compared and contrasted for gene expression among the parthenotes, as well as against zygotic blastocysts.

2022-02-01 | GSE111112 | GEO

Gene expression profile changes during blastocysts hatching of embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

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Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine blastocysts produced in vitro.

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| S-EPMC4768781 | biostudies-literature

Gene Expression Profiling of Blastocysts Reveals Early Differences Associated with Parthenogenetic Embryos

Project description:The goal of this experiment is to test the differences between parthenogenetic and normal fertilized embryos at the blastocyst stage. Keywords: gene expression assay for comparison purposes

2010-03-08 | GSE7399 | GEO

Broad, ectopic expression of the sperm protein, PLCZ1 induces parthenogenesis and ovarian tumours in mice

Project description:Mammalian metaphase II (mII) exit and embryogenesis are induced at fertilization by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1). Meiotic progression can also be triggered without sperm, as in parthenogenesis, although the classic mouse in vivo parthenogenetic model, LT/Sv, fails in meiosis I due to an unknown molecular etiology. We here dissect PLCZ1 specificity and function in vivo and address its ability to interfere with maternal meiotic exit. Wild-type mouse Plcz1 expression was restricted to post-pubertal testes, and the brains of both sexes, with region-specifying elements mapping to a 4.1 kb Plcz1 promoter fragment. When broad ectopic PLCZ1 expression was forced in independent transgenic lines, they initially appeared healthy. Their oocytes underwent unperturbed meiotic maturation to mII but subsequently exhibited autonomous intracellular free calcium oscillations, second polar body extrusion, pronucleus formation and parthenogenetic development. Transfer of transgenic cumulus cell nuclei into wild-type oocytes induced activation and development, demonstrating a direct effect of PLCZ1 analogous to fertilization. Whilst Plcz1 transgenic males remained largely asymptomatic, females developed abdominal swellings caused by benign ovarian teratomas that were under-represented for paternally- and placentally-expressed transcripts. Plcz1 was not over-expressed in the ovaries of LT/Sv or in human germline ovarian tumours. The narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors. This work characterizes a novel model in which parthenogenesis and tumourigenesis follow full meiotic maturation and are linked to fertilization by PLCZ1. Keywords: miRNA profiling; expression profiling of mouse miRNAs_SampleB Fourteen samples were analyzed for the study.

2007-10-01 | E-GEOD-4822 | biostudies-arrayexpress

Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions

2012-09-21 | GSE41043 | GEO

Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions

Project description:Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expression profile of rabbit parthenote embryos cultured under in vivo conditions using microarray analysis. Transcriptional microarray study that compares gene expression of 6 day parthenote rabbit embryos to their in vivo counterparts. Biological replicates used: 4 replicates for control embryos, 4 replicates for parthenote embryos

2012-09-21 | E-GEOD-41043 | biostudies-arrayexpress

buffalo parthenogenesis TMT LC-MS/MS

Project description:Oocytes act as the genetic vector for zygote reprogramming, and stores a large amount of material that is required to regulate embryogenesis. However, to date, there is no report on the dynamic changes of maternal proteins and genes that occur during the early stages of the embryo, particularly studies that use proteomic techniques in buffalos. Here, an integrated single-cell RNA sequencing transcriptomic and quantitative proteomic analysis were employed to systematically explore the dynamic function of maternally-expressed proteins in parthenogenesis model of buffalo.The proteome of the buffalo was quantitatively analyzed during parthenogenesis of mature oocytes and the two-cell stage embryo. Of 1,908 quantified proteins, 123 differed significantly. The transcriptome was analyzed 8 stages (GV, MII, 2-cell,4-cell,8-cell,16-cell,morula,blastocyst)of buffalo using the single-cell RNA sequencing, and a total of 3567 unique genes were identified to be differently expressed between all consecutive stages of pre-implantation development. Bioinformatics studies and validated results indicated that maternal expression of the proteins possibly plays a role in the formation of cellular junctions firstly after parthenogenetic activation and the “maternal-to-zygotic transition” (MZT) process exists during parthenogenesis and occur between the 8-cell to 16-cell stage.

2018-07-17 | PXD008549 | Pride

Amplification of Oocytes through Parthenogenesis

Project description:We asked whether oocyte number could be amplified through parthenogenesis of mouse oocytes, without requiring creation of a paternal genome and a genetically unique genome. Parthenotes develop to a blastocyst-like stage, and from this parthenogenetic ESCs (pESCs) can be derived with high efficiency. Like ESCs, pESCs maintain unlimited self-renewal and pluripotency, as well as germline competence. Further, we demonstrate that their expression pattern of imprinted maternal genes resembles that observed in oocytes. pESCs can be directed to differentiate into primordial germ cell-like cells (PGCLCs) and form oocytes that produce fertile pups and reconstitute ovarian endocrine functions. The transcriptome and imprinting pattern of PGCLCs differentiated from pESCs more closely approximate those of in vivo produced embryonic PGCs, than PGCLCs produced from ESCs. Parthenogenesis offers a promising route for deriving PGCLCs and amplifying oocytes by faithfully maintaining maternal genes, without fertilization.

2019-11-28 | GSE141103 | GEO

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