Dataset Information


Transcriptional analysis of adaptation to high glucose concentration in Zymomonas mobilis

ABSTRACT: High glucose concentrations were desirable for ethanol fermentation of Zymomonas mobilis, but it can lead to decrease in ethanol production and productivity. Sorbitol as a compatible solute can be absorbed or synthesized to counteract the detrimental osmotic stress caused from external high glucose concentrations by Z. mobilis. Currently, molecular mechanisms of tolerance to high glucose concentrations and sorbitol promoting ethanol fermentation are still unclear for Z. mobilis. To better understand mechanisms with which high concentrations of glucose and sorbitol affect physiology and metabolism of Z. mobilis ATCC31821 (ZM4), the global transcriptional responses of ZM4 to the challenge of high glucose concentration and sorbitol were profiled using whole genome microarray analysis. Swings J, Deley J. Bacterial Rev. 1977, 41(1): 1-46. Loos H, Kramer R, Sahm H and Sprenger GA. J Bacteriol. 1994, 176(24):7688–7693. Overall design: Gene expression analysis was performed using twelve independent microarray experiments (four time point reactions × three biological replicates), representing four patterns of growth conditions from ZM4 cultures. Each microarray measures the expression level of 1,918 genes from ZM4 ATCC31821 and the associated plasmids, with three-fold technical redundancy.

INSTRUMENT(S): NimblenGen ZM4 12*135K

SUBMITTER: kun zhang  

PROVIDER: GSE49620 | GEO | 2014-10-14



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Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis.

Zhang Kun K   Shao Huanhuan H   Cao Qinghua Q   He Ming-Xiong MX   Wu Bo B   Feng Hong H  

Applied microbiology and biotechnology 20150113 4

The ethanologenic bacterium Zymomonas mobilis is usually tolerant to high concentrations of glucose. The addition of sorbitol decreases the lag phase and increases ethanol yield and productivity of the bacteria in high glucose concentrations. The molecular mechanisms of adaptation to high glucose concentrations and the effect of sorbitol are still unclear. In this study, microarray analysis was used to study the global transcriptional adaptation responses of Z. mobilis to high glucose concentrat  ...[more]

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