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Effect of overexpression of OsTGAP1 in chitin oligosaccharide elicitor-treated rice cells

ABSTRACT: To see the effect of overexpression of the bZIP transcription factor OsTGAP1, we compared the gene expression profiles in wild type rice cells and OsTGAP1-overexpressing rice cells.

ORGANISM(S): Oryza sativa

PROVIDER: GSE53417 | GEO | 2014/04/01

SECONDARY ACCESSION(S): PRJNA231941

REPOSITORIES: GEO

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Effect of overexpression of OsTGAP1 in chitin oligosaccharide elicitor-treated rice cells

Project description:To see the effect of overexpression of the bZIP transcription factor OsTGAP1, we compared the gene expression profiles in wild type rice cells and OsTGAP1-overexpressing rice cells. Expression profiling in the wild type rice cells and OsTGAP1-overexpressing rice cells treated with chitin oligosaccharide for 0, 6, and 24 h was compared using two-color method with four biological replicates.

2014-04-01 | E-GEOD-53417 | biostudies-arrayexpress

Chitin oligosaccharide elicitor-responsive genes in suspension cultured rice cells

Project description:Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 6 and 24 h after treatment using rice 44k microarray.

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Chitin oligosaccharide elicitor-responsive genes in suspension cultured rice cells

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The function of CEBiP in the chitin elicitor signaling in rice cells

Project description:To see the function of CEBiP in the chitin elicitor signaling in rice cells, a plasmid for the gene-specific knock-down by RNA interference (RNAi) was constructed by using a sequence for the 3’-terminal region of CEBiP and transformed into the rice cells by using Rhizobium radiobacter (Agrobacterium tumefaciens). chitinoligomer was added (or not added) to the medium. For the control of them, rice cells that non transformed wild type was used. Keywords: genetic modification

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The function of OsCERK1 in the chitin elicitor signaling in Rice

Project description:To see the function of OsCERK1 receptor-like kinase in the chitin elicitor signaling in Rice, we compared the gene expression profiles in the chitin oligosaccharide treated cultured rice cells of vector control and OsCERK1 knock-down mutant (RNAi). Keywords: Defense response 1,Chitin oligosaccharide treatment (vector control), 2,Chitin oligosaccharide treatment (vector control) color swap, 3,Chitin oligosaccharide treatment (OsCERK1 RNAi), 4,Chitin oligosaccharide treatment (OsCERK1 RNAi) color swap

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Effect of overexpression of the OsWRKY53 or phospho-mimicking mutant of OsWRKY53 in rice

Project description:To see the effect of phosphorylation of OsWRKY53, we compared the gene expression profiles in the rice plants overexpressing native OsWRKY53 or a phospho-mimicking mutant of OsWRKY53.

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Chitin oligosaccharide-responsive genes in suspension-cultured rice cells

Project description:Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 1-12 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with chitin oligosaccharide for 1, 2, 4, 6, 8 and 12 h was compared with that in untreated control using two-color method with two biological replicates.

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WRKY type transcription factor OsWRKY28 overexpression effect on rice leaves 48 h after Magnaporthe Oryzae infection

Project description:Analysis of transgenic rice overexpressing OsWRKY28, a WRKY type transcription factor. Results provide insight into the role of OsWRKY28 in the defense signaling against rice blast fungus.

2012-09-30 | GSE39635 | GEO

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