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Azaspiracid_jurkat_time series experiment

ABSTRACT: Azaspiracid-1 (AZA-1) is a marine phycotoxin that accumulates in shellfish and known to cause severe gastrointestinal intoxications in humans. As the mechanism of action of AZA-1 is currently unknown, human DNA microarrays were used to profile gene expression in human lymphocyte T cells following AZA-1 exposure. Highly significant early (1 hr) genes consisted of a T cell specific transcription factor, membrane proteins, receptors, and inflammatory genes. At 4 hr, responding genes included transcription factors and cell growth genes, in addition to 16 genes involved in fatty acid and cholesterol synthesis. Similarly, at 24 hr, 17 of the top 35 signature genes were involved in fatty acid and cholesterol synthesis. Gene Map Annotator and Pathway Profiler software confirmed cell signaling effects targeted toward lipid biosynthesis pathways. The transcriptional profile induced by AZA-1 shared high similarity to expression profiles of in vitro cholesterol synthesis inhibitor studies and in vitro and in vivo cholesterol synthesis gene knockout studies, suggesting a common transcriptional response and possible mechanism of action. Keywords: Time course; AZA treatment vs. vehicle control

ORGANISM(S): Homo sapiens

PROVIDER: GSE5346 | GEO | 2008/02/19

SECONDARY ACCESSION(S): PRJNA96205

REPOSITORIES: GEO

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Transcription profiling of human jurkat cell line treated with the marine phyotoxin Azaspiracid-1 (AZA)

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