Transcriptomics,Genomics

Dataset Information

13

Fgd5 indentifies hematopoietic stem cells in the murine bone marrow is is not required for definitive hematopoiesis


ABSTRACT: Analysis of purified murine hematopoietic samples Overall design: 122 Affymetrix MOE430 2.0 arrays generated from purified steady-state murine hematopoietic cell types were re-normalized together using gcRMA in R. Their GSE Series numbers are as follows: GSE34723, GSE11982, GSE7302, GSE8407,GSE9717, GSE10627, GSE10246, GSE12581, GSE6506, GSE11110, GSE12001, GSE9810, GSE4142, GSE7764, GSE9810, GSE6875, GSE6681

SUBMITTER: Isabel Beerman 

PROVIDER: GSE56952 | GEO | 2014-04-22

SECONDARY ACCESSION(S): PRJNA245048

REPOSITORIES: GEO

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Publications

Fgd5 identifies hematopoietic stem cells in the murine bone marrow.

Gazit Roi R   Mandal Pankaj K PK   Ebina Wataru W   Ben-Zvi Ayal A   Nombela-Arrieta César C   Silberstein Leslie E LE   Rossi Derrick J DJ  

The Journal of experimental medicine 20140623 7


Hematopoietic stem cells (HSCs) are the best-characterized tissue-specific stem cells, yet experimental study of HSCs remains challenging, as they are exceedingly rare and methods to purify them are cumbersome. Moreover, genetic tools for specifically investigating HSC biology are lacking. To address this we sought to identify genes uniquely expressed in HSCs within the hematopoietic system and to develop a reporter strain that specifically labels them. Using microarray profiling we identified s  ...[more]