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SaOS-2 transfected with CD99 in differentiation medium for 14 days [miRNA]

ABSTRACT: We explored the transcriptional modification induced by CD99 transfection in the osteosarcoma cell lines SaOS-2 after 0, 7 and 14 days in differentiation medium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE61929 | GEO | 2015/06/01

SECONDARY ACCESSION(S): PRJNA262695

REPOSITORIES: GEO

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SaOS-2 transfected with CD99 in differentiation medium for 14 days [total RNA]

Project description:We explored the transcriptional modification induced by CD99 transfection in the osteosarcoma cell lines SaOS-2 after 0, 7 and 14 days in differentiation medium.

2015-06-01 | GSE61928 | GEO

SaOS-2 transfected with CD99 in differentiation medium for 14 days

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-06-01 | GSE61930 | GEO

The HLA peptide repertoire of SaOS-2 cell line transfected with TP53 protein

Project description:Analysis of the immunopeptidome of Human Leukocyte Antigen(HLA)class I of SaOS-2 osteosarcoma cells transfected with pBR322 vector only, and 2 mutants of TP53 protein namely R175H and R273H.

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Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α

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2014-02-27 | GSE54942 | GEO

DNase-seq profiling of Saos-2 and U2OS cell lines

Project description:We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.

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CD99 suppresses osteosarcoma cell migration by favouring the oncosuppressor N-cadherin/ B-catenin signalling pathway in contrast to ezrin: the central role of ROCK2

Project description:To determine the molecular basis for CD99 function in osteosarcoma, gene expression profile of U-2/CD99 wild-type(wt) expressing cell lines were compared with U-2 OS parental cells.

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Proteolytic processing of CD99

Project description:The adhesion molecule CD99 is essential for transendothelial migration (TEM) of leukocytes. Here we demonstrate by biochemical and cellular assays that CD99 undergoes ectodomain shedding by the metalloprotease meprin β and subsequent intramembrane proteolysis by γ-secretase. The cleavage site in CD99 was identified by mass spectrometry within an acidic region highly conserved through different vertebrate species.

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Saos-2 vs. Saos-2-2 cells

Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression.

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CD99 inhibits neural differentiation of human Ewing sarcoma cells

Project description:Ewing sarcoma (EWS) is an aggressive bone tumor of uncertain cellular origin. CD99 is a membrane protein that is expressed in most cases of EWS, although its function in the disease is unknown. Here we have shown that endogenous CD99 expression modulates EWS tumor differentiation and malignancy. We determined that knocking down CD99 expression in human EWS cell lines reduced their ability to form tumors and bone metastases when xenografted into immunodeficient mice and diminished their tumorigenic characteristics in vitro. Further, reduction of CD99 expression resulted in neurite outgrowth and increased expression of β-III tubulin and markers of neural differentiation. Analysis of a panel of human EWS cells revealed an inverse correlation between CD99 and H-neurofilament expression, as well as an inverse correlation between neural differentiation and oncogenic transformation. As knockdown of CD99 also led to an increase in phosphorylation of ERK1/2, we suggest that the CD99-mediated prevention of neural differentiation of EWS occurs through MAPK pathway modulation. Together, these data indicate a new role for CD99 in preventing neural differentiation of EWS cells and suggest that blockade of CD99 or its downstream molecular pathway may be a new therapeutic approach for EWS.

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McGill EMC Community projects Release 7 for cell line "SaOS-2" - samples

Project description:Complete reference epigenome (as defined by IHEC) of a SaOS-2 cell line with osteosarcoma.

| EGAD00001007678 | EGA