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Legionella ITS oligonucleotide microarray

ABSTRACT: In this report, we have developed a rapid oligonucleotide microarray detection technique to identify the most common ten Legionella spp.. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven air conditioner-condensed water samples with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed interestingly that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp..

ORGANISM(S): Legionella maceachernii Legionella micdadei Legionella pneumophila Legionella Fluoribacter gormanii Legionella bozemanae Legionella anisa Legionella jordanis Legionella longbeachae Fluoribacter dumoffii Legionella fairfieldensis

PROVIDER: GSE61962 | GEO | 2014/10/04

SECONDARY ACCESSION(S): PRJNA263045

REPOSITORIES: GEO

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