Genomics

Dataset Information

29

Genome-wide expression profiling after Allyl Alcohol treatment in Saccharomyces cerevisiae


ABSTRACT: Allyl alcohol is a highly toxic industrial chemical used as a synthetic substrate, and as an herbicide in agriculture. It is evident that Allyl alcohol is metabolized by alcohol dehydrogenases (ADH) to the highly toxic Acrolein. Acrolein is a simple unsaturated aldehyde, ubiquitous environmental pollutant, endogenous metabolite and major constituent of cigarette smoke. Acrolein is highly electrophilic in nature and has strong reactivity towards nucleophiles present in cell such as amino acids, proteins and DNA. Several studies have tried to explore the molecular mechanisms of acrolein cytotoxicity. In continuation, we attempted to investigate the global transcriptional response of yeast cells upon treatment with 0.4mM Allyl alcohol (Acrolein) using oligonucleotide microarray assay. Also, we investigated for functionally enriched pathways based on the altered gene expression profiles after Allyl alcohol treatment. The microarray data were validated by quantitative real-time PCR. Overall design: Wild-type yeast (W1588-4c) cells were grown to exponential phase and the cells were left untreated (Control) or treated with a dose of 0.4mM Allyl Alcohol (AA) for 3h. Two independent experiments were performed for each experiment (Control or AA). Total RNAs were extracted and hybridized on Affymetrix microarrays.

REANALYSED by: GSE62400

INSTRUMENT(S): [Yeast_2] Affymetrix Yeast Genome 2.0 Array

SUBMITTER: Raghuvir singh Tomar  

PROVIDER: GSE62399 | GEO | 2015-04-30

SECONDARY ACCESSION(S): PRJNA264016

REPOSITORIES: GEO

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Publications

Molecular cytotoxicity mechanisms of allyl alcohol (acrolein) in budding yeast.

Golla Upendarrao U   Bandi Goutham G   Tomar Raghuvir S RS  

Chemical research in toxicology 20150513 6


Allyl alcohol (AA) is one of the environmental pollutants used as a herbicide and industrial chemical. AA undergoes enzymatic oxidation in vivo to form Acrolein (Acr), a highly reactive and ubiquitous environmental toxicant. The exposure to AA/Acr has detrimental effects on cells and is highly fatal. In corroboration to the current literature describing AA/Acr toxicity, this study aimed to investigate the molecular cytotoxicity mechanisms of AA/Acr using budding yeast as a eukaryotic model organ  ...[more]

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