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CD44s transfection in HEK cells

ABSTRACT: In order to understand the transcriptional effects of CD44s expression in a cell line that does not express CD44 in its native form we transfected CD44s into HEK cells and measured the transcriptional chances compared to native HEK cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE63862 | GEO | 2014/12/05

SECONDARY ACCESSION(S): PRJNA269283

REPOSITORIES: GEO

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CD44s transfection in HEK cells

Project description:In order to understand the transcriptional effects of CD44s expression in a cell line that does not express CD44 in its native form we transfected CD44s into HEK cells and measured the transcriptional chances compared to native HEK cells Three biological replicates from each condition (native HEK cells and CD44s transfected cells) were measured using Affymetrix arrays

2014-12-05 | E-GEOD-63862 | biostudies-arrayexpress

Human normal oral keratinocytes (NOK) siRNA and Human epidermal keratinocytes (HEK) overexpression RNA-Seq

Project description:Transcriptome of si-RNA transfected NOK cells and adenovirus-transduced HEK cells

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RNA-Sequencing of STING competent and STING-KO cells after transfection with dacA (Listeria monocytogenes)

Project description:HEK-Blue™ ISG Cells and HEK-Blue™ ISG-KO-STING Cells (Invivogen) were transfected with dacA (Listeria monocytogenes) for 12 and 24 hours. Through this approach, we described the STING-dependent and STING-independent transcriptional response to dacA. Our analysis reveals a STING-dependent enrichment of interferon stimulated genes beginning 12 hours post-transfection, and a STING-independent enrichment of genes associated with protein translation and oxidative phosphorylation.

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Homo sapiens

Project description:CD44s transfection in HEK cells

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HEK-293 cells: Control cells transfected with hRIZ2 and rRIZ1

Project description:In order to evaluate the effects of RIZ2 overexpression on gene expression, a pilot expression study was performed through microarrays analysis. We compared the differential gene expression between HEK-293 cells overexpressing human RIZ2 (pEGFP-hRIZ2) and rat RIZ1 (pEGFP-rRIZ1) versus control cells transfected with the E-GFP empty vector alone (pEGFP).

2020-12-09 | GSE150031 | GEO

Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16

2013-11-21 | GSE52546 | GEO

Real-time quantitative PCR analysis of Wild-type p53 (Wtp53) and Mutant p53 (Mutp53) transiently overexpressed in HEK-293T cells

Project description:To identify a novel lncRNA regulated by mutp53. Wtp53 and 5 different hotspots mutation of p53 were transiently transfected into HEK-293T cells and compared with plasmid vector (pcDNA6A-Flag).

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Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16

Project description:HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 with the goal of comparing the global gene expression profiles in the Aire and MBD-VP16 groups We used microarrays to detail the global gene expression profile. HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 and 72 hrs post transfection total RNA was collected for microarray analysis.

2013-11-21 | E-GEOD-52546 | biostudies-arrayexpress

Gene expression data from HEK-293T cells transfected with miR-194 mimics

Project description:Nuclear factor κB (NF-κB) pathway plays an important role in hepatocellular carcinoma (HCC) progression. miR-194 was previously shown to reduce the induction of NF-κB activity upon addition of tumor necrosis factor α (TNFα). To clarify the molecular mechanism responsible for the effect of miR-194 on NF-κB pathway, mRNA microarray assays were performed to identify the genes that were suppressed by miR-194. HEK-293T cells transfected with miR-194 mimics were cultured for RNA extraction and hybridization on Affymetrix mRNA microarrays. These were compared against the control, which were HEK-293T cells transfected with negative control mimics.

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Transcription Level Response of HEK-293T Cells to RfA1 Expression

Project description:We transfected HEK-293T cells to express RfA1. The RNA-Seq results indicates that Genes evolved in biological processes such as “regulation of microtubule cytoskeleton organization”, “microtubule polymerization or depolymerization”, “microtubule nucleation” were found to respond to RfA1 expression. Correspondingly, these microtubule relevant genes were also sorted to cellular component items, including “spindle microtubule”, “spindle pole centrosome”, “mitotic spindle”, “spindle” , which indicates the tight relationship between RfA1 and microtubules.

2021-11-01 | GSE186861 | GEO

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