Project description:To examine the Boea hygrometrica genome methylation landscape and assess its functional significance during dehydration, we generated the first single-base resolution genome methylation maps for leaf tissues of hydrated, dehydrating to 70% RWC and dehydrating to 10% RWC (desiccating). The overall density of methyl-cytosine was not broadly altered by dehydration. There is, however, a small increase in intensity both for the whole genome and regionally during the first dehydration step (70% RWC) returning to near hydrated levels as leaves desiccate. We identified 5575 differentially methylated region-related genes during dehydration. The finding may provide an important clue for exploring molecular mechanisms and the regulation of desiccation tolerance at the whole-genome level.
Project description:It has been thought that epigenetic changes underlie the evolutionary divergence of phenotype between closely related species. To study differences in DNA methylation between humans and chimanzees, we collected methylated DNAs from age- and gender-matched PBL samples of the two species, and deeply sequenced. The map counts were then converted to MEDIPS scores in 100-bp bins, which are related to absolute methylation levels.