Dataset Information


HRI Protects Erythroid Precursors in iron deficiency and beta-Thalassemia by Maintaining GATA-1 and Fog-1 Expression

ABSTRACT: Heme-regulated eIF2α kinase (HRI) is essential for the survival of erythroid precursors in iron and heme deficiency and it also plays a protective role in red blood cell diseases of erythroid protoporphyria and β-thalassemia. In this study, we demonstrated for the first time the impairment of GATA-1 and Fog-1 expressions in iron deficiency and the impairment of GATA-1 expression in β-thalassemia. Furthermore, HRI is necessary to maintain the GATA-1/Fog-1 induced functions in erythroid differentiation, cell cycle and cell survival by sustaining both expressions of GATA-1 and Fog-1 in iron deficiency and in β-thalassemia. Keywords: Genetic modification Overall design: Three replicates of gene chip profiling were performed for each condition, Wt+Fe, Wt-Fe, Ko+Fe and Ko-Fe.


INSTRUMENT(S): [HG-U133A] Affymetrix Human Genome U133A Array

SUBMITTER: Sanchita Bhattacharya  

PROVIDER: GSE6808 | GEO | 2009-06-30



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Haem-regulated eIF2alpha kinase is necessary for adaptive gene expression in erythroid precursors under the stress of iron deficiency.

Liu Sijin S   Bhattacharya Sanchita S   Han Anping A   Suragani Rajasekhar N V S RN   Zhao Wanting W   Fry Rebecca C RC   Chen Jane-Jane JJ  

British journal of haematology 20080728 1

Haem-regulated eIF2alpha kinase (HRI) is essential for the regulation of globin gene translation and the survival of erythroid precursors in iron/haem deficiency. This study found that that in iron deficiency, fetal definitive erythropoiesis is inhibited at the basophilic erythroblast stage with increased proliferation and elevated apoptosis. This hallmark of ineffective erythropoiesis is more severe in HRI deficiency. Microarray gene profiling analysis showed that HRI was required for adaptive  ...[more]

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