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Transcriptomic impact of AWR5 effector expression in S. cerevisiae

ABSTRACT: The type III effector (T3E) AWR5 from the phytopathogen Ralstonia solanacearum causes disease in many plants relevant in agriculture. Heterologous expression in yeast of AWR5 from an integrated tetO promoter caused strong growth inhibition. In this series of experiments we investigate the possible changes in the transcriptional profile in a time-course (2, 4 and 6 h) response to AWR5 production.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE70202 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA287835

REPOSITORIES: GEO

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Transcriptomic impact of AWR5 effector expression in S. cerevisiae

Project description:The type III effector (T3E) AWR5 from the phytopathogen Ralstonia solanacearum causes disease in many plants relevant in agriculture. Heterologous expression in yeast of AWR5 from an integrated tetO promoter caused strong growth inhibition. In this series of experiments we investigate the possible changes in the transcriptional profile in a time-course (2, 4 and 6 h) response to AWR5 production. Yeast strains were grown overnight in rich YPD medium with doxycycline 15 Âµg/ml (repressing conditions), then normalized to OD600=0.05 and grown resumed in YPD+dox (non-inducing conditions) or YPD (inducing conditions). Samples were taken at 2, 4 or 6 hours for each condition. Two biological replicates were made for each time point.

2015-12-31 | E-GEOD-70202 | biostudies-arrayexpress

Expression of AWR5 in wild type and cdc55 deletion mutant at 6 h of induction

Project description:Expressing the type III effector AWR5 of the phytopathogen Ralstonia solanacearum in yeast cells causes cessation of growth and transcriptional changes reminiscent of inhibition of the TOR pathway. Deletion of cdc55, a regulatory subunit of the PP2A Ser/Thr protein phosphatase eliminates the strong growth defect caused by AWR5 expression. This experiments aims to identify the impact of the cdc55 mutation in the transcriptome of AWR5-expressing cells. induction of AWR5 in JA-100 CDC55 tetO:AWR5-GFP and JA-100 cdc55 tetO:AWR5-GFP

2015-12-31 | E-GEOD-70331 | biostudies-arrayexpress

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Ralstonia solanacearum effector-specific effect on Medicago truncatula trancriptome

Project description:affy_ralstonia_peeters_medicago - We have identified two essential virulence determinants (GALA7, a type III secretion effector and HpaP, a chaperone-like protein) of Ralstonia solanacearum for the infection and colonisation of the host plant Medicago truncatula. The scope of this project is to identify the GALA7 and HpaP-specific transcriptome alteration. For this purpose wild type and mutant infected root material (13h and 72h postinfection) will be analysed on M. truncatula affymetrix chips. Medicago truncatula (A17 line) are grown in vitro on Farheus medium (with Nitrogen source) plantlets are inoculated with water R. solanacearum wt, gala7 and hpap mutants, and root tips are collected at 13h and 72h postinoculation. Experiment was performed 3 times independently. 4 bacteria conditions x 2 harvest times x 3 biological repeats = 24 samples Keywords: gene knock out,normal vs disease comparison,time course,treated vs untreated comparison 24 arrays - Medicago

2012-12-01 | E-GEOD-21985 | biostudies-arrayexpress

Ralstonia solanacearum effector-specific effect on Medicago truncatula trancriptome

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Proteome of potato responding to Ralstonia solanacearum

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Project description:Examination of modified bases on the genome of Ralstonia solanacearum UY031

2016-12-29 | GSE92982 | GEO

Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain GMI1000 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain GMI1000 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 5061 genes from Ralstonia solanacearum strain GMI1000 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

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