Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time course of the response to ACTH in pig: biological and transcriptomic study

ABSTRACT: To explore individual variation in the biological response to cortisol, the main stress hormone, in pigs, we have developed a microarray analysis to study the kinetics of the response to an ACTH injection measured at 4 time points. ACTH is a hormone secreted by the pituitary gland and triggering the release of cortisol by the adrenal glands in response to a stress. The objective was to identify genes up- or down-regulated during the stress response triggered by cortisol in the whole blood.

ORGANISM(S): Sus scrofa

PROVIDER: GSE71207 | GEO | 2015/07/23

SECONDARY ACCESSION(S): PRJNA290582

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Time course of the response to ACTH in pig: biological and transcriptomic study

Project description:To explore individual variation in the biological response to cortisol, the main stress hormone, in pigs, we have developed a microarray analysis to study the kinetics of the response to an ACTH injection measured at 4 time points. ACTH is a hormone secreted by the pituitary gland and triggering the release of cortisol by the adrenal glands in response to a stress. The objective was to identify genes up- or down-regulated during the stress response triggered by cortisol in the whole blood. To explore individual variation in the biological response to cortisol, whole blood from 30 juvenile Large White (LW) pigs was collected at 4 time point ( t = 0, +1 h, +4 h, +24 h) for a total of 120 samples. All pigs were females and were raised in two batches. Arrays were designed such that all observations of a sole individual were kept on the same array, with two individuals (one from each batch) on one array. This design secured the kinetics of the response for each individual and prevented confounding effects between batch and array. After quality and control, 120 samples were kept for normalization and analyses (represented here).

2015-07-23 | E-GEOD-71207 | biostudies-arrayexpress

DH06 Adrenal ACTH Sus scrofa

Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig

2008-01-07 | GSE8377 | GEO

DH06 Adrenal ACTH Sus scrofa

Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 Âµg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80Â°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples

2010-06-29 | E-GEOD-8377 | biostudies-arrayexpress

ACTH-independent macronodular adrenocortical hyperplasia (AIMAH)

Project description:ACTH-independent macronodular adrenal hyperplasia (AIMAH) is a clinically and genetically heterogeneous disorder that can be associated with aberrant hormone receptors. Whole-genome expression profiling was analyzed in samples of different nodules from a patient with AIMAH. Total RNA obtained from adrenal nodules were compared to those samples obtained normal adrenal pools

2010-10-30 | E-GEOD-25031 | biostudies-arrayexpress

ACTH-independent macronodular adrenocortical hyperplasia (AIMAH)

2010-10-30 | GSE25031 | GEO

Transcription controls growth, cell kinetics and cholesterol supply to sustain ACTH responses.

Project description:Investigating a mouse model of Cushing syndrome, synthetic adrenocorticotropic hormone ACTH (Synacthen) was infused for two weeks to markedly increase adrenal mass and plasma corticosterone levels. Gene expression in adrenals was measured to look for changes associated with the adrenal cellular phenotype.

2017-10-11 | E-MTAB-5704 | biostudies-arrayexpress

Genome-wide analysis of adult adrenocortical cells gene expression after 48 h ACTH treatment

Project description:Analysis of ACTH-regulation on adrenocortical cells at gene expression level. The hypothesis tested in the present study was that ACTH increases chronic cell growth and steroidogenesis in adrenal glands by changing the gene expression profile. Results provide important information of the response of adrenocortical cells gene expression to chronic ACTH treatment. Total RNA obtained from primary cultured adult adrenal cells subjected to 48 hours in vitro treatment of ACTH compared to untreated control cell.

2010-07-08 | E-GEOD-20669 | biostudies-arrayexpress

Affymetrix SNP6 array data for ACTH-Independent Macronodular Adrenal Hyperplasia (AIMAH) samples

Project description:AIMAH is an ACTH-independent bilateral enlargement of the adrenal cortex occuring during adulthood. The enlargment is related to the growth of multiple benign nodules. This condition is associated with various degrees of cortisol hypersecretion. The occurence of several nodules in both adrenals, and the existence of familial forms, suggest the existence of a germline genetic predisposition. To find the gene(s), the aim of the project was to look for recurrent chromosomal alterations in the AIMAH nodules. Extensive mapping of somatic gains, losses and copy-neutral loss of heterozygosity (LOH) was performed with Affymetrix SNP6 arrays. A copy neutral LOH of 16p, occuring in 7 of 26 patients, was one of the only recurrent alterations, pointing towards a candidate gene in this region. Of note this condition differs from the congenital adrenal hyperplasias, related to genetic alterations of steroidogenesis (the latter is an ACTH dependent adrenal hyperplasia). Affymetrix SNP6 arrays were performed according to the manufacturer's directions on DNA extracted from cryopreserved tumor samples or peripheral blood samples.

2013-11-28 | GSE42171 | GEO

Genome-wide analysis of adult adrenocortical cells gene expression after 48 h ACTH treatment

2010-06-16 | GSE20669 | GEO

Affymetrix SNP6 array data for ACTH-Independent Macronodular Adrenal Hyperplasia (AIMAH) samples

2013-11-28 | E-GEOD-42171 | biostudies-arrayexpress