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Gene expression profile of liver tissue from carbon tetrachloride (CCl4)-treated mouse cultured ex vivo


ABSTRACT: Gene-expression profiles of liver tissue of cabon tetrachloride (CCl4)-treated and control mice were obtained before and after organotypic ex vivo tissue culture. keywords: liver tissue, mouse, gene-expression microarray, Illumina, ex vivo tissue culture Overall design: Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Strain A/J male mice (Jackson Laboratory, Bar Harbor, ME) were treated three times a week for 18 weeks with either 0.1cc of a 40 percent solution of CCl4 (Sigma) in olive oil or with vehicle control by oral gavage. Mice were sacrificed and the liver was harvested for RNA extraction (day 0) or organotypic ex vivo tissue culture. Fresh liver tissues were sectioned into 300mm-thick slices and cultured for 24h (day 1) or 48h (day 2) with DMEM/F12 media (Sigma) supplemented with 10% FBS, 100U/mL penicillin, 100mg/mL streptomycin, and 2.5mg/mL amphotericin B. RNA was isolated using TRIzol reagent, and subjected to expression profiling.

INSTRUMENT(S): Illumina MouseRef-8 v2.0 expression beadchip

ORGANISM(S): Mus Musculus

SUBMITTER: Yujin Hoshida  

PROVIDER: GSE71379 | GEO | 2016-12-16

SECONDARY ACCESSION(S): PRJNA291061

REPOSITORIES: GEO

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Publications

Molecular Liver Cancer Prevention in Cirrhosis by Organ Transcriptome Analysis and Lysophosphatidic Acid Pathway Inhibition.

Nakagawa Shigeki S   Wei Lan L   Song Won Min WM   Higashi Takaaki T   Ghoshal Sarani S   Kim Rosa S RS   Bian C Billie CB   Yamada Suguru S   Sun Xiaochen X   Venkatesh Anu A   Goossens Nicolas N   Bain Gretchen G   Lauwers Gregory Y GY   Koh Anna P AP   El-Abtah Mohamed M   Ahmad Noor B NB   Hoshida Hiroki H   Erstad Derek J DJ   Gunasekaran Ganesh G   Lee Youngmin Y   Yu Ming-Lung ML   Chuang Wan-Long WL   Dai Chia-Yen CY   Kobayashi Masahiro M   Kumada Hiromitsu H   Beppu Toru T   Baba Hideo H   Mahajan Milind M   Nair Venugopalan D VD   Nair Venugopalan D VD   Lanuti Michael M   Villanueva Augusto A   Sangiovanni Angelo A   Iavarone Massimo M   Colombo Massimo M   Llovet Josep M JM   Subramanian Aravind A   Tager Andrew M AM   Friedman Scott L SL   Baumert Thomas F TF   Schwarz Myron E ME   Chung Raymond T RT   Tanabe Kenneth K KK   Zhang Bin B   Fuchs Bryan C BC   Hoshida Yujin Y  

Cancer cell 20161201 6


Cirrhosis is a milieu that develops hepatocellular carcinoma (HCC), the second most lethal cancer worldwide. HCC prediction and prevention in cirrhosis are key unmet medical needs. Here we have established an HCC risk gene signature applicable to all major HCC etiologies: hepatitis B/C, alcohol, and non-alcoholic steatohepatitis. A transcriptome meta-analysis of >500 human cirrhotics revealed global regulatory gene modules driving HCC risk and the lysophosphatidic acid pathway as a central chemo  ...[more]

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