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Human pulmonary microvascular endothelial cells (HPMECs): Control siRNA vs HSP27 siRNA

ABSTRACT: Transcriptional profiling of HPMECs comparing Control siRNA treated with HSP27 siRNA

ORGANISM(S): Homo sapiens

PROVIDER: GSE72485 | GEO | 2015/08/29

SECONDARY ACCESSION(S): PRJNA294143

REPOSITORIES: GEO

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Human pulmonary microvascular endothelial cells (HPMECs): Control siRNA vs HSP27 siRNA

Project description:Transcriptional profiling of HPMECs comparing Control siRNA treated with HSP27 siRNA Two-condition experiment, control siRNA vs HSP27 siRNA ; 3 controlsiRNA transfected replicates , 3HSP27 siRNA replicates. Replicates were pooled and hybridized to a single array.

2015-08-29 | E-GEOD-72485 | biostudies-arrayexpress

HSP27 3A vs WT

Project description:human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides. Keywords: parallel sample

2004-04-09 | GSE1292 | GEO

Multiple waves of transcriptome changes during extended hypoxic induction in human pulmonary microvascular endothelial cells

Project description:We profile the expression pattern of human pulmonary microvascular endothelial cells (HPMECs) at different time points of hypoxic stress. Through mRNA-seq, we identify functional waves of minor gene up-regulation at 8 and 24h hypoxia exposure followed by a massive wave of transcriptional activation after 48 hours. By weighted gene co-expression network analysis, we identify hub genes that likely play central roles in hypoxia transcription program. Strikingly, these hub genes included a prominent group of lincRNAs, suggesting non-coding RNAs may also have pivotal roles in the hypoxia regulatory circuit. HPMECs share a core hypoxia signature profile, but with some notably differences, indicating a portion of HPMECs hypoxia response is cell-specific. Collectively, our study comprehensive surveys the hypoxia transcriptome, and provides insight into the temporal dynamics of hypoxia transcriptional response. Time-course expression profiling of HPMECs exposed to hypoxia

2015-12-31 | E-GEOD-53510 | biostudies-arrayexpress

Transcription profiling by array of castration-resistant prostate cancer PC-3 cells treated with Hsp27-siRNA or control siRNA to study the role of Heat shock protein (Hsp) 27 in splicing

Project description:In order to investigate the involvement of Hsp27 in splicing, we performed a whole-genome exonic expression profiling of the castration-resistant prostate cancer PC-3 cells treated by Hsp27-siRNA or CTL-siRNA (both in biological duplicates).

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HSP27 3A vs WT

2010-06-09 | E-GEOD-1292 | biostudies-arrayexpress

Transcriptomics revealed sex differences in human pulmonary microvascular endothelial cells in normoxia and hypoxia

Project description:To investigate sex differences at the transcriptome level in human pulmonary microvascular endothelial cells (HPMECs) from healthy male and female donors basally (in normoxia) and in hypoxic conditions. RNA-seq was performed on male (n=3) and female (n=4) HPMECs that were cultured in conditions of physiological shear stress (PMID: 36730645) in normoxia (21% O2) or in hypoxia (1% O2) for either 24 or 48 hours.

2023-12-01 | GSE233547 | GEO

Multiple waves of transcriptome changes during extended hypoxic induction in human pulmonary microvascular endothelial cells

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human pulmonary artery (endothelial and smoorh muscle) cells : control vs ARSB-siRNA transfected

Project description:Transcriptional profiling of human pulmonary artery endothelial (HAPEC) and smooth muscle (PASM) cells comparing control untreated cells with cells transfected with ARSB-siRNA. Goal was to determine the effects of silenced of arylsufatase B on global gene expression in HAPEC and PASM cells.

2018-10-01 | GSE115268 | GEO

Homo sapiens

Project description:Human pulmonary microvascular endothelial cells (HPMECs): Control siRNA vs HSP27 siRNA

| PRJNA294143 | ENA

Identification and Functional Analysis of Long Non-coding RNAs in Human Pulmonary Microvascular Endothelial Cells Subjected to Cyclic Stretch

Project description:To screen the differential expression of mRNAs and lncRNAs in Human pulmonary microvascular endothelial cells (HPMECs) subjected to cyclic stretch, we performed transcriptome profiling using Affymetrix Human Transcriptome Array 2.0.

2021-02-17 | GSE166772 | GEO

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