Transcriptomics,Genomics

Dataset Information

52

Transcriptomic effects of TNFa in primary fibroblast-like synovial cell cultures


ABSTRACT: Gene expression profiles in synovial biopsies from patients with rheumatoid arthritis (RA) display a high level of plasticity related to disease activity and response to therapy. In order to identify TNFa-dependent genes in ex vivo RA or other synovial biopsies, we generated the present set of data using primary fibroblast-like synovial cell (FLS) cultures incubated overnight in the presence or the absence of TNFa. Overall design: FLS were purified from knee synovial biopsies obtained in one patient with RA, and one patient with osteoarthritis. For this experiment, cells between passage 7 and 9 were cultured in the presence or the absence of 10 ng/ml human recombinant TNFa. After overnight incubation, cells were detached, total RNA was extracted, amplified and labeled using a standard Affymetrix procedure, and hybridized on HGU133 Plus2.0 slides.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Bernard Robert Lauwerys  

PROVIDER: GSE73764 | GEO | 2015-10-07

SECONDARY ACCESSION(S): PRJNA297858

REPOSITORIES: GEO

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Publications

Higher expression of TNFα-induced genes in the synovium of patients with early rheumatoid arthritis correlates with disease activity, and predicts absence of response to first line therapy.

De Groof Aurélie A   Ducreux Julie J   Humby Frances F   Nzeusseu Toukap Adrien A   Badot Valérie V   Pitzalis Costantino C   Houssiau Frédéric A FA   Durez Patrick P   Lauwerys Bernard R BR  

Arthritis research & therapy 20160120


IL6-related T cell activation and TNFα-dependent cell proliferation are major targets of therapy in the RA synovium. We investigated whether expression of these pathways in RA synovial biopsies is associated with disease activity and response to therapy.Correlation and gene set enrichment studies were performed using gene expression profiles from RA synovial biopsies. Immunostaining experiments of GADD45B and PDE4D were performed on independent additional sets of early untreated RA samples, obta  ...[more]

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