Transcriptomics,Genomics

Dataset Information

36

ANTIBIOTICS OVERPRODUCTION IN STREPTOMYCES COELICOLOR A3(2) MEDIATED BY PHOSPHOFRUCTOKINASE A2 DELETION


ABSTRACT: Gene expression analysis of S. coelicolor M145 and the delta_pfkA2 mutant. RNA samples were taken in the early exponential phase during growth in fermentors in defined mineral medium. Keywords: cell type, gene expression, genetic modification Overall design: 5 Streptomyces ceolicolor Extracts (3 M145 WILD TYPE, 2 M145 Delta_pfkA2 MUTANT) were hybridised with dye swaps on 4 arrays: - Sco8.26 (M145 RNA No. 1 cy5 + M145 delta_pfkA2 RNA No. 1 cy3) - Sco8.28 (M145 delta_pfkA2 RNA No. 2 cy5 + M145 RNA No. 3 cy3) - Sco8.31 (M145 RNA No. 2 cy5 + M145 delta_pfkA2 RNA No. 2 cy3) - Sco8.37 (M145 delta_pfkA2 RNA No. 1 cy5, M145 RNA No. 2 cy3).

INSTRUMENT(S): UNIS_S COELICOLOR_SCo3_8149

SUBMITTER: Giles Velarde 

PROVIDER: GSE7686 | GEO | 2008-05-01

SECONDARY ACCESSION(S): PRJNA99439

REPOSITORIES: GEO

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Publications

Antibiotic overproduction in Streptomyces coelicolor A3 2 mediated by phosphofructokinase deletion.

Borodina Irina I   Siebring Jeroen J   Zhang Jie J   Smith Colin P CP   van Keulen Geertje G   Dijkhuizen Lubbert L   Nielsen Jens J  

The Journal of biological chemistry 20080707 37


Streptomycetes are exploited for production of a wide range of secondary metabolites, and there is much interest in enhancing the level of production of these metabolites. Secondary metabolites are synthesized in dedicated biosynthetic routes, but precursors and co-factors are derived from the primary metabolism. High level production of antibiotics in streptomycetes therefore requires engineering of the primary metabolism. Here we demonstrate this by targeting a key enzyme in glycolysis, phosph  ...[more]

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