Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of shoot apices of 35S::WOX1:GR seedlings transiently treated with DEX

ABSTRACT: The WOX1 transcription factor is a multifunctional regulator of lateral-organ development that acts as a transcriptional repressor. WOX1 promotes lamina growth, controls marginal tissue differentiation and is involved in establishment and maintenance of the adaxial-abaxial pattern from the middle domain of leaf primordia. To identify the WOX1 downstream genes, we performed a microarray analysis of shoot apices of transgenic Arabidopsis thaliana lines harboring 35S::WOX1:glucocorticoid receptor (GR) in which the WOX1 function was temporarily enhanced by dexamethasone (DEX).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE79647 | GEO | 2018/05/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression data from 35S:miR396b plants

Project description:Transcript profile of apices of 20 days-old Arabidopsis plants over expressing miR396b. We used ATH1 Affymetrics microarrays to obtain the transcription profile of plants overexpressing miR396b. We compared the transcriptome of 35S:miR396b plants vs wild-type plants (Col-0 accession), using ATH1 Affymetrics microarrays. For these experiments we collected vegetative apices together with the leaf primordia from plants of 20 days growth in short-day. For each genotype (Col-0, 35S:miR396b) we collected two samples.

2014-07-16 | E-GEOD-53839 | biostudies-arrayexpress

662107

Project description:Adaxial and Abaxial Leaf Metagenomes

| PRJNA591421 | ENA

CRABS CLAW positively regulates genes involved in the establishment of both abaxial and adaxial identity in Arabidopsis

Project description:CRABS CLAW (CRC) encodes a transcription factor of the plant-specific YABBY class in the model plant Arabidopsis thaliana. This gene is highly expressed in the abaxial (external) domain of the gynoecium wall and contributes to the establishment of abaxial-adaxial (external-internal) polarity in that tissue. Here we derive a list of putative target genes of CRC, which include AUXIN RESPONSE FACTOR4 (ARF4) and ASSYMETRIC LEAVES1 (AS1), both of which are known to be involved in the establishment of abaxial-adaxial polarity in lateral organs. ETTIN (ETT), which is partially redundant with ARF4, was not identified as a direct target of CRC, and this observation led us to predict that crc/ett double mutants might resemble ett/arf4 double mutants, which show a very strong breakdown of abaxial-adaxial polarity in the gynoecium wall. We have confirmed this prediction by constructing double mutants using several available mutant alleles of crc, ett and arf4. Interestingly, AS1 plays a role in the establishment of adaxial tissue identity in lateral organs, though its expression is not restricted to the adaxial domain. The observed positive regulation of AS1 by CRC may thus occur very early in gynoecium development, before CRC expression becomes polarised, or at later stages of development, specifically in the abaxial domain.

2021-03-05 | GSE14659 | GEO

Methylation Changes in Soybean Early Maturation Seed Compartments Using LCM

Project description:What methylation changes are occurring in different compartments of early maturation stage seed largely remains unknown. To uncover the possible role of DNA methylation in different compartments of early maturation stage seed, we characterized the methylome of two major compartments in embryonic cotyledon: cotyledon abaxial parenchyma (EM-COT-ABPY) and cotyledon adaxial parenchyma (EM-COT-ADPY) using Illumina sequencing. Illumina sequencing of bisulfite-converted genomic DNA from cotyledon abaxial parenchyma (EM-COT-ABPY) and cotyledon adaxial parenchyma (EM-COT-ADPY) compartments.

2012-05-11 | E-GEOD-37893 | biostudies-arrayexpress

Inflorescence apices: rtr-2 versus wild type samples

Project description:Transcriptional profiling of inflorescence apices from wild type and rtr-2 mutant plants . Goal was to determine the differentially expressed genes between wild type and mutant plants in inflorescence apices.

2014-12-31 | GSE60169 | GEO

Genetic networks regulating structure and function of the maize shoot apical meristem

Project description:The shoot apical meristem (SAM) of plants, a specialized stem cell niche at the growing shoot tip, integrates developmental and environmental signals to direct the initiation and patterning of new organs such as leaves. Its activity throughout the plant's lifetime is tightly controlled. To gain insight into gene regulatory networks behind stem cell maintenance and organogenesis, we generated a high-resolution gene expression atlas of distinct domains and cell types within the vegetative shoot apex of 14 day-old maize B73 seedlings using laser microdissection and RNA deep sequencing. Data for 9 domains was previously released under SRA: SRP101301. This release adds data for biological duplicate samples collected for the SAM central zone, and the adaxial and abaxial sides of leaf primordia.

2019-10-02 | GSE137715 | GEO

Transcriptome of ang3 mutant shoot apices

Project description:The role of translation in the regulation of higher plant growth and development is not well understood. Mutational analysis is a powerful tool to identify and study the function of genes related to a biological process, such as growth. Here we analyzed functionally the angusta3 (ang3) narrow leaf mutant. The ANG3 gene was cloned by fine-mapping combined with candidate gene sequencing and it corresponded to the ribosomal protein gene RPL5B. Based on amino acid sequence homology, promoter DNA sequence homology and in silico gene expression analysis, RPL5B was found to be putatively functionally redundant with RPL5A. The morphological analysis of ang3 mutants showed that the leaf lamina area was significantly reduced from the third rosette leaf on, mainly because of decreased width. Cellular analysis of the abaxial epidermal cell layer of the third leaf indicated that the cell number in the mutant was similar to that of the wild type, but the cell size was significantly reduced. We postulate that the reduced cell expansion in the epidermis contributes to the narrow shape of ang3 leaves. Growth was also significantly impaired in hypocotyls and primary roots, hinting at a general role for RPL5B in organ growth, unrelated to dorsi-ventral axis formation. Comparison of the transcriptome of the shoot apices of the mutant and the wild type revealed a limited number of differentially expressed genes, such as MYB23 and MYB5, of which the lower expression in the ang3 mutant correlated with reduced trichome density. Our data suggest that translation is an important level of control of growth and development in plants.

2009-10-14 | GSE18492 | GEO

Expression data from 35S:miR396b plants

2014-07-16 | GSE53839 | GEO

Gene expression analysis of the inflorescence of 35S::SUP-GR after Mock and DEX treatment

Project description:Identification of putative downstream genes of SUP with the functional inducible line 35S::SUP-GR

2018-01-31 | GSE92729 | GEO

Transcription profiling of Arabidopsis wild type and myo mutant shoot apices

Project description:Transcription profiling of shoot apices of the myo mutant was compared with shoot apices of WT (background Ler).

2009-07-22 | E-MEXP-1735 | biostudies-arrayexpress