Transcriptomics,Genomics

Dataset Information

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Genome wide gene-expression analysis of the spider mite Tetranychus urticae after transfer from their common host (bean) to either cotton, maize or soy


ABSTRACT: A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes. Overall design: Three major comparisons were investigated: 4 biological replicates of soy adaptation (London-SOY vs London), 4 biological replicates of maize adaptation (London-MAIZE vs London) and 3 biological replicates of cotton adaptation (London-COTTON vs London).

INSTRUMENT(S): Agilent-033850 Tetranychus urticae 63K array

SUBMITTER: Nicky Wybouw 

PROVIDER: GSE80337 | GEO | 2016-10-10

SECONDARY ACCESSION(S): PRJNA318632

REPOSITORIES: GEO

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Publications


The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions. Here, the proteomic composition of saliva from T. urticae l  ...[more]

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