Transcriptomics

Dataset Information

55

Resting and IL2 activated human natural killer cells


ABSTRACT: The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Keywords: resting and IL2 activated NK cells(time series) Overall design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

ORGANISM(S): Homo sapiens  

SUBMITTER: Javeed Iqbal   

PROVIDER: GSE8059 | GEO |

SECONDARY ACCESSION(S): PRJNA100891

REPOSITORIES: GEO

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