Genomics

Dataset Information

38

Quantitative transcriptomic analysis metabolic mutant of Clostrdidium acetobutylicum ATCC824 under different physiological conditions


ABSTRACT: Transcriptomic analysis was applied to acidogenic, solventogenic and alcohologenic steady-state C. acetobutylicum cells for understanding the regulation of the metabolism of this organism. Used microarray is Clostridium acetobutylicum ATCC824 transcriptional 44k v4, correspondance to GPL10908 Overall design: Phosphate-limited continuous cultures of C. acetobutylicum were performed under acidogenesis (pH 6.3, 995 mM of carbon from glucose), solventogenesis (pH 4.4, 995 mM of carbon from glucose) and alcohologenesis (pH 6.3, 498 mM of carbon from glucose and 498 mM of carbon from glycerol). Samples were collected for three biological replicates from one control strain and three metabolic mutants, respectively. Total RNA of 36 samples was extracted using an RNeasy Midi kit (Qiagen, Courtaboeuf, France) following the manufacturer's instructions with the supplementation of DNase treatment using RNase-Free DNase Set (Qiagen). Agilent microarray for Clostridium acetobutylicum ATCC 824 (4x44K AMADID 024247) was used. The RNAs were labeled with a Low Input Quick Amp Labeling kit and hybridized following a one-color microarray-based gene expression analysis protocol.

INSTRUMENT(S): Clostridium acetobutylicum ATCC 824 transcriptional 44k v4

SUBMITTER: Philippe Soucaille 

PROVIDER: GSE81273 | GEO | 2016-05-11

SECONDARY ACCESSION(S): PRJNA321166

REPOSITORIES: GEO

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Publications

Cap0037, a Novel Global Regulator of Clostridium acetobutylicum Metabolism.

Nguyen Ngoc-Phuong-Thao NP   Linder Sonja S   Flitsch Stefanie K SK   Schiel-Bengelsdorf Bettina B   Dürre Peter P   Soucaille Philippe P  

mBio 20161004 5


An operon comprising two genes, CA_P0037 and CA_P0036, that encode proteins of unknown function that were previously shown to be highly expressed in acidogenic cells and repressed in solventogenic and alcohologenic cells is located on the pSOL1 megaplasmid of Clostridium acetobutylicum upstream of adhE2 A CA_P0037::int (189/190s) mutant in which an intron was inserted at position 189/190 in the sense strand of CA_P0037 was successfully generated by the Targetron technique. The resultant mutant s  ...[more]

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