HS 5hr
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE870 | GEO | 2004/02/24
SECONDARY ACCESSION(S): PRJNA89837
REPOSITORIES: GEO
Similar Datasets
Project description:embryos exposed to 43°C for 15 minutes (HS) followed by 1 hour at 37°C Keywords: other
2004-02-24 | GSE869 | GEO
Project description:Analysis of embryos exposed to either: [1] 40 uM 4-hydroperoxycyclophosphamide for 1 or 5 hours at 37°C [2] 43°C heat shock for 15 minutes followed by 1 or 5 hours at 37°C This SuperSeries is composed of the following subset Series: GSE866: CP 1hr GSE869: HS 1hr GSE870: HS 5hr GSE888: CP 5hr
2004-02-23 | E-GEOD-905 | biostudies-arrayexpress
Project description:Analysis of embryos exposed to either: [1] 40 uM 4-hydroperoxycyclophosphamide for 1 or 5 hours at 37°C [2] 43°C heat shock for 15 minutes followed by 1 or 5 hours at 37°C This SuperSeries is composed of the SubSeries listed below.
2004-02-24 | GSE905 | GEO
Project description:Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed. The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then the Qiagen RNeasy Midi Kit with some modifications within the manufacturers´ protocol and the Qiagen RNase-Free DNase Set were applied. 6 µg of the total RNA per sample was used for each microarray experiments. The indirect labelling by the tyramide-signal-amplification method (MicromaxTM TSATM labelling and detection Kit from Perkin Elmer life sciences) was used to increase the Cy3 and Cy5 signals of microarray detection. Each cDNA containing Biotin- and Fluorescein-nucleotides respectively was purified with a QIAquick PCR purification kit and suspended in 11 µl of the formamide containing hybridization buffer. The slides were hybridized at 42°C over night under a cover slip. The microarrays were scanned by the Axon 4000B scanner; image intensity data were extracted and analysed with GenePix® Pro 6.0 software. Data from different scans of Dye-swap experiment were extracted by GenePix Pro 6.0 software, normalized and united. An outliertest has been applied in order to find outliers amongst the gene replicats. Subsequently, a t-test (1% and 5% probability of error) has been used in order to find regulated genes. Keywords: sorted yeast cells Microarray experiments were realised with dye swap.
2008-12-11 | E-GEOD-12369 | biostudies-arrayexpress
Project description:Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed. The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then the Qiagen RNeasy Midi Kit with some modifications within the manufacturers´ protocol and the Qiagen RNase-Free DNase Set were applied. 6 µg of the total RNA per sample was used for each microarray experiments. The indirect labelling by the tyramide-signal-amplification method (MicromaxTM TSATM labelling and detection Kit from Perkin Elmer life sciences) was used to increase the Cy3 and Cy5 signals of microarray detection. Each cDNA containing Biotin- and Fluorescein-nucleotides respectively was purified with a QIAquick PCR purification kit and suspended in 11 µl of the formamide containing hybridization buffer. The slides were hybridized at 42°C over night under a cover slip. The microarrays were scanned by the Axon 4000B scanner; image intensity data were extracted and analysed with GenePix® Pro 6.0 software. Data from different scans of Dye-swap experiment were extracted by GenePix Pro 6.0 software, normalized and united. An outliertest has been applied in order to find outliers amongst the gene replicats. Subsequently, a t-test (1% and 5% probability of error) has been used in order to find regulated genes. Keywords: sorted yeast cells
2008-12-12 | GSE12369 | GEO
Project description:embryos exposed to 40 uM 4-hydroperoxycyclophosphamide (4CP) for 5 hours at 37°C Keywords: other
2004-02-24 | GSE888 | GEO
Project description:First, each sample (30.00 mg) was weighed and transferred to a 2 mL centrifuge tube, followed by adding two 5 mm magnetic beads, an appropriate amount of SDS-containing protein lysis buffer, and 1× Cocktail (protease inhibitor) with a final concentration of EDTA. The samples were disrupted and lysed using an automatic grinder, then centrifuged at 25,000g at 4℃ for 15 minutes to collect the supernatant. Next, DTT was added to the supernatant to a final concentration of 10 mM, and the mixture was incubated in a 37℃ water bath for 30 minutes. Subsequently, IAM was added to a final concentration of 55 mM, and the samples were placed in a dark room for 45 minutes. Five volumes of pre-cooled acetone were then added, and the mixture was stored in a -20℃ refrigerator for 2 hours, followed by centrifugation at 25,000g at 4℃ for 15 minutes to discard the supernatant. The precipitate was air-dried, and an appropriate amount of SDS-free protein lysis buffer was added. An automatic grinder was used to promote protein dissolution, and the mixture was centrifuged again at 25,000g at 4℃ for 15 minutes. The resulting supernatant was the final protein solution, which was used for subsequent quantification and analysis.
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