Transcriptomics,Genomics

Dataset Information

165

Regulated inflammation and lipid metabolism in colon mRNA expressions of obese germfree mice responding to Enterobacter cloacae B29 combined with the high fat diet


ABSTRACT: Increased evidences demonstrated that gut microbiota targeted diet intervention can alleviate obesity and related metabolic disorders. The underlying mechanism of interactions among diet, microbiota and host still remains unclear. Enterobacter cloacae B29, an endotoxin-producing strain dominated in the gut of a morbidly obese volunteer (weight 174.8 kg, BMI 58.8 kg m-2) was isolated and transplanted to germfree mice. Using deep mRNA sequencing technology, we compared different gene expression profiles in the colon samples of the germfree mice before and after treated with B29 and/or high fat diet (HFD) and identified 279 differential expressed genes in total, including up-regulated genes Apoa4, Ido1, Cyp4a10, and down-regulated genes Cyp2e1, Cyp26b1, Akr1b7, Adipoq, Cyp1a1, Apoa1, Npc1l1, Tff2, Apoc1, Ctla2a, Mttp, Lpl. Fifty-nine GO biological processes and five KEGG pathways, particularly the Peroxisome proliferator-activated receptors (PPARs) signaling pathway, were significantly enriched in response to HFD+B29, which were mainly relevant to inflammation and the metabolism of lipid, lipoprotein and sterols. These functional changes were consistent with the developed obesity, insulin-resistance, and aggravated inflammatory conditions of the HFD+B29 mice. This work provides insight into the gene expression changes in response to HFD+B29, helping to understand the mechanism of the interactions among HFD, B29 and the germfree mice. Overall design: Four groups(n=6) of germfree mice treated with: NCD+LB, NCD+B29, HFD+LB, HFD+B29

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: menghui zhang  

PROVIDER: GSE87027 | GEO | 2017-02-22

SECONDARY ACCESSION(S): PRJNA343248

REPOSITORIES: GEO

altmetric image

Publications

Regulated Inflammation and Lipid Metabolism in Colon mRNA Expressions of Obese Germfree Mice Responding to Enterobacter cloacae B29 Combined with the High Fat Diet.

Yan Huiying H   Fei Na N   Wu Guojun G   Zhang Chenhong C   Zhao Liping L   Zhang Menghui M  

Frontiers in microbiology 20161108


Increased evidences have demonstrated that gut microbiota targeted diet intervention can alleviate obesity and related metabolic disorders. The underlying mechanism of interactions among diet, microbiota, and host still remains unclear. Enterobacter cloacae B29, an endotoxin-producing strain dominated in the gut of a morbidly obese volunteer (weight 174.8 kg, BMI 58.8 kg m-2) was isolated and transplanted to germfree mice (inoculated 1010 cells of B29 per day for 1 week). Using deep mRNA sequenc  ...[more]

Similar Datasets

2010-08-20 | E-GEOD-19038 | ArrayExpress
| GSE19038 | GEO
2012-09-08 | E-GEOD-34154 | ArrayExpress
2014-03-28 | E-GEOD-56257 | ArrayExpress
| GSE68441 | GEO
2013-07-01 | E-GEOD-42840 | ArrayExpress
| GSE42840 | GEO
| GSE81318 | GEO
2015-02-04 | E-GEOD-65557 | ArrayExpress
2013-09-10 | E-GEOD-37095 | ArrayExpress