Transcriptomics,Genomics

Dataset Information

152

Gene expression changes in dauer defective strains


ABSTRACT: Animals change developmental fates in response to external cues. In the nematode Caenorhabditis elegans, unfavorable environmental conditions induce a state of diapause known as dauer by inhibiting the conserved DAF-2 insulin-like signaling (ILS) pathway through incompletely understood mechanisms. We previously established a role for the C. elegans dosage compensation protein DPY-21 in the control of dauer arrest and DAF-2 ILS. Here we show that dosage compensation acts through the histone H4 lysine 20 methyltransferase SET-4 to promote dauer arrest in part by repressing the X-linked ins-9 gene, which encodes a new agonist insulin-like peptide (ILP) expressed specifically in the paired ASI sensory neurons that are required for dauer bypass. ins-9 repression in dauer-constitutive mutants requires DPY-21, SET-4, and the FoxO transcription factor DAF-16, which is the main target of DAF-2 ILS. In contrast, autosomal genes encoding major agonist ILPs that promote reproductive development are not repressed by DPY-21, SET-4, or DAF-16/FoxO. Our results implicate the dosage compensation machinery as a sensory rheostat that reinforces developmental fates in response to environmental cues by modulating autocrine and paracrine DAF-2 ILS. Overall design: 35 total samples, 7 strains collected in 5 replicates each. C elegans larvae were grown for 24 hr at 25C (developmental stage L2D in eak-7;akt-1 background). L2D animals were compared to wild type animals and dauer-defective compound mutants deficient in daf-16, dpy-21, set-4 or daf-12.

INSTRUMENT(S): Illumina HiSeq 2500 (Caenorhabditis elegans)

SUBMITTER: Richard C McEachin  

PROVIDER: GSE89295 | GEO | 2017-02-28

SECONDARY ACCESSION(S): PRJNA351259

REPOSITORIES: GEO

altmetric image

Publications

A histone H4 lysine 20 methyltransferase couples environmental cues to sensory neuron control of developmental plasticity.

Delaney Colin E CE   Chen Albert T AT   Graniel Jacqueline V JV   Dumas Kathleen J KJ   Hu Patrick J PJ  

Development (Cambridge, England) 20170216 7


Animals change developmental fates in response to external cues. In the nematode Caenorhabditis elegans, unfavorable environmental conditions induce a state of diapause known as dauer by inhibiting the conserved DAF-2 insulin-like signaling (ILS) pathway through incompletely understood mechanisms. We have previously established a role for the C. elegans dosage compensation protein DPY-21 in the control of dauer arrest and DAF-2 ILS. Here, we show that the histone H4 lysine 20 methyltransferase S  ...[more]

Similar Datasets

| GSE84581 | GEO
2015-11-09 | E-GEOD-67650 | ArrayExpress
2006-01-06 | E-SMDB-3596 | ArrayExpress
2006-02-02 | E-GEOD-4164 | ArrayExpress
| GSE87741 | GEO
2013-06-18 | E-GEOD-43086 | ArrayExpress
2012-11-01 | E-GEOD-41943 | ArrayExpress
| GSE69329 | GEO
| PRJNA184364 | ENA
2015-08-27 | E-GEOD-72426 | ArrayExpress