Transcriptomics,Genomics

Dataset Information

57

Global miRNA expression profiles of fibroblast-like synoviocytes in patients with rheumatoid arthritis and osteoarthritis


ABSTRACT: Fibroblast-like synoviocyte (FLS) constitutes a major cell subset of rheumatoid arthritis (RA) joint. Dysregulation of microRNAs (miRNAs) contributes to FLS activation in the context of chronic inflammation. However, functional association of the miRNAs-targets relationships characterizing FLS phenotypes in RA has not been fully elucidated yet. Thus, we uncovered the novel miRNA-target interactions characterizing pathologic phenotypes of RA-FLS. We performed microarray analyses of miRNA in RA- and osteoarthritis (OA) FLS with or without interleukin-1β (IL-1β) stimulation. The miRNA-target prediction and network model-ing were performed using TargetScan and Cytoscape. Identified miRNA-target relationships and their cellular functions were validated in vitro. Overall design: To identify miRNA expression signatures of FLS from rheumatoid arthritis patients, we isolated FLS from synovial tissues of RA and osteoarthritis (OA) patients and stimulated FLS with IL1β. Finally, we analyzed gene expression profiles of both IL1β-stimulated RA-FLS and OA-FLS

INSTRUMENT(S): Agilent-029297 Human miRNA Microarray (Feature Number version)

SUBMITTER: Bong-Ki Hong  

PROVIDER: GSE91026 | GEO | 2017-10-31

SECONDARY ACCESSION(S): PRJNA356702

REPOSITORIES: GEO

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Publications

MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis.

Hong Bong-Ki BK   You Sungyong S   Yoo Seung-Ah SA   Park Dohyun D   Hwang Daehee D   Cho Chul-Soo CS   Kim Wan-Uk WU  

Experimental & molecular medicine 20170804 8


Fibroblast-like synoviocytes (FLSs) constitute a major cell subset of rheumatoid arthritis (RA) synovia. Dysregulation of microRNAs (miRNAs) has been implicated in activation and proliferation of RA-FLSs. However, the functional association of various miRNAs with their targets that are characteristic of the RA-FLS phenotype has not been globally elucidated. In this study, we performed microarray analyses of miRNAs and mRNAs in RA-FLSs and osteoarthritis FLSs (OA-FLSs), simultaneously, to validat  ...[more]

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