Dataset Information


T47D_MTVL treatment with JAK/STAT inhibitor AG490 with/without R5020

ABSTRACT: Gene expression of the breast cancer cell line T47D-MTVL induced by progestin R5020, in the absense or presence of the JAK/STAT signaling pathway inhibitor AG490. Keywords: Gene expression - T47D-MTVL- hormonal treatment+inhibitors comparison Overall design: Two biological replicates of breast cancer cell line T47D-MTVL cell line samples were treated with eiter progestin R5020 or with the drug vehicle Ethanol. One hour before this treatment, samples were treated with the JAK/STAT inhibitor AG490. Cell line sample was labeled with Cy5 and hybridized against Universal Human Reference RNA (Stratagene) labeled with Cy3.

INSTRUMENT(S): CRG Human Breast Cancer Array v4.0-0.8K


PROVIDER: GSE9286 | GEO | 2007-10-12



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Progesterone induction of the 11beta-hydroxysteroid dehydrogenase type 2 promoter in breast cancer cells involves coordinated recruitment of STAT5A and progesterone receptor to a distal enhancer and polymerase tracking.

Subtil-Rodríguez Alicia A   Millán-Ariño Lluís L   Quiles Ignacio I   Ballaré Cecilia C   Beato Miguel M   Jordan Albert A  

Molecular and cellular biology 20080331 11

Steroid hormone receptors regulate gene expression, interacting with target DNA sequences but also activating cytoplasmic signaling pathways. Using the human 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) gene as a model, we have investigated the contributions of both effects on a human progesterone-responsive promoter in breast cancer cells. Chromatin immunoprecipitation has identified two different mechanisms of hormone-induced progesterone receptor (PR) recruitment to the 11beta-HSD  ...[more]

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