Transcriptomics,Genomics

Dataset Information

31

Endomyocardial biopsies with different acute rejection gradings


ABSTRACT: Our goals were to determine whether (i) gene expression patterns in biopsies grouped by acute rejection grade (AR) would be consistent; (ii) a characteristic gene expression profile would be associated with ISHLT Grade 0, 1A & 1B (equivalent to 1R on recently revised criteria) and ISHLT grade 3A & 3B (equivalent to 2R & 3R on recently revised criteria); (iii) gene expression patterns emerging from the analysis would provide insights into mechanisms of AR. The samples used for gene expression profiling were endomyocardial biopsies (EMB) with different acute rejection gradings. The EMB (n=2726) were obtained from the right site of the interventricular septum of heart transplant recipients (n=2423) and graded according to the guidelines of the International Society for Heart and Lung Transplantation (ISHLT, grade 0, n=9; grade1A, n=5; 1B, n=5; 3A, n=4; 3B, n=43). Selection criteria for inclusion of patients in this analysis required the availability of appropriately stored EMB samples for RNA extraction; absence of any infection that required treatment with an antimicrobial drug at anytime during the posttransplant course of the patient; absence of prior AR rejection of any grade; absence of hemodynamic compromise at the time of EMB; use of standard immunosuppressive regimen; and the absence of HLA antibodies prior to transplant. Overall design: An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.

INSTRUMENT(S): Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)

SUBMITTER: Cecile Holweg  

PROVIDER: GSE9377 | GEO | 2008-10-18

SECONDARY ACCESSION(S): PRJNA103085

REPOSITORIES: GEO

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Publications

Identification and classification of acute cardiac rejection by intragraft transcriptional profiling.

Holweg Cécile T J CT   Potena Luciano L   Luikart Helen H   Yu Tianwei T   Berry Gerald J GJ   Cooke John P JP   Valantine Hannah A HA   Mocarski Edward S ES  

Circulation 20110509 20


Treatment of acute rejection (AR) in heart transplantation relies on histopathological grading of endomyocardial biopsies according to International Society for Heart and Lung Transplantation guidelines. Intragraft gene expression profiling may be a way to complement histological evaluation.Transcriptional profiling was performed on 26 endomyocardial biopsies, and expression patterns were compared with the 1990 International Society for Heart and Lung Transplantation AR grades. Importantly, tran  ...[more]

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