Transcriptomics,Genomics

Dataset Information

45

Autonomous TNF is critical for monocyte survival in steady state and inflammation in vivo


ABSTRACT: Monocytes are circulating mononuclear phagocytes, poised to extravasate to sites of inflammation and differentiate into monocyte-derived macrophages and dendritic cells. Tumor necrosis factor (TNF) and its receptors are upregulated during monopoiesis and expressed by circulating monocytes, as well as effector monocytes infiltrating certain sites of inflammation, such the spinal cord during experimental autoimmune encephalomyelitis (EAE). Using competitive in vitro and in vivo assays, we show here that monocytes deficient for TNF or TNF receptors are outcompeted by their wild-type counterpart. Moreover, monocyte autonomous TNF is critical for the function of these cells, as TNF ablation in monocytes / macrophages, but not in microglia delayed the onset of EAE in challenged animals and was associated with reduced acute spinal cord infiltration of Ly6Chi effector monocytes. Collectively, our data reveal a previously unappreciated critical cell-autonomous role of TNF on monocytes for their survival, maintenance and function. Overall design: BM precursors and steady state monocytes are taken and compared with effector spinal cord monocytes in EAE day 9

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Steffen Jung  

PROVIDER: GSE94546 | GEO | 2017-04-19

SECONDARY ACCESSION(S): PRJNA371454

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE94546_Compare_monocytes.txt.gz Txt
GSE94546_Expression_data.txt.gz Txt
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Publications

Autonomous TNF is critical for in vivo monocyte survival in steady state and inflammation.

Wolf Yochai Y   Shemer Anat A   Polonsky Michal M   Gross Mor M   Mildner Alexander A   Yona Simon S   David Eyal E   Kim Ki-Wook KW   Goldmann Tobias T   Amit Ido I   Heikenwalder Mathias M   Nedospasov Sergei S   Prinz Marco M   Friedman Nir N   Jung Steffen S  

The Journal of experimental medicine 20170322 4


Monocytes are circulating mononuclear phagocytes, poised to extravasate to sites of inflammation and differentiate into monocyte-derived macrophages and dendritic cells. Tumor necrosis factor (<i>TNF</i>) and its receptors are up-regulated during monopoiesis and expressed by circulating monocytes, as well as effector monocytes infiltrating certain sites of inflammation, such as the spinal cord, during experimental autoimmune encephalomyelitis (EAE). In this study, using competitive in vitro and  ...[more]

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