Transcriptomics,Genomics

Dataset Information

167

A Comparison of the Sexually Dimorphic Dexamethasone Transcriptome in Mouse Cerebral Cortical and Hypothalamic Embryonic Neural Stem Cells


ABSTRACT: We used RNA-Seq analysis to identify the sexually dimorphic dexamethasone regulated gene expression profile in primary mouse embryonic cerebral cortical and hypothalamic neural progenitor/stem cells. Overall design: RNAseq on primary E14.5 mouse cerebral cortical and hypothalamic (hypo) neurosphere cultures. 8 conditions - Male Cortex Dex, Male Cortex Ethanol vehicle (EtOH), Female Cortex Dex, Female Cortex EtOH, Male Hypo Dex, Male Hypo EtOH, Female Hypo Dex and Female Hypo EtOH. There are 3 biological replicates for each condition and all the 24 samples were run on two lanes (technical duplicates).

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Uma Chandran  

PROVIDER: GSE95363 | GEO | 2017-05-26

SECONDARY ACCESSION(S): PRJNA376745

REPOSITORIES: GEO

altmetric image

Publications

Research Resource: The Dexamethasone Transcriptome in Hypothalamic Embryonic Neural Stem Cells.

Frahm Krystle A KA   Peffer Melanie E ME   Zhang Janie Y JY   Luthra Soumya S   Chakka Anish B AB   Couger Matthew B MB   Chandran Uma R UR   Monaghan A Paula AP   DeFranco Donald B DB  

Molecular endocrinology (Baltimore, Md.) 20151125 1


Exposure to excess glucocorticoids during fetal development has long-lasting physiological and behavioral consequences, although the mechanisms are poorly understood. The impact of prenatal glucocorticoids exposure on stress responses in juvenile and adult offspring implicates the developing hypothalamus as a target of adverse prenatal glucocorticoid action. Therefore, primary cultures of hypothalamic neural-progenitor/stem cells (NPSCs) derived from mouse embryos (embryonic day 14.5) were used  ...[more]

Similar Datasets

| GSE111945 | GEO
2015-12-09 | E-GEOD-55113 | ArrayExpress
2018-10-18 | GSE121497 | GEO
2014-03-19 | E-GEOD-53028 | ArrayExpress
2013-01-30 | E-TIGR-103 | ArrayExpress
2013-01-30 | E-TIGR-104 | ArrayExpress
2005-10-01 | GSE3396 | GEO
| GSE109796 | GEO
2013-04-26 | E-GEOD-40582 | ArrayExpress
2016-06-10 | E-GEOD-75330 | ArrayExpress