Genomics

Dataset Information

167

Maternal obesity induced mouse embryonic defects derive from Stella insufficiency in oocytes


ABSTRACT: In order to obtain genome-wide profiles, base-resolution methylomes of zygotes were generated using the bisulfite sequencing (BS-seq) method for small samples. We find that global loss of DNA methylation during zygotic development in HFD mice. A total of 412 DMRs were identified, of which 294 were hypomethylated (hypo-DMRs; 71.4%) and 118 were hypermethylated (hyper-DMRs; 28.6%) (Fig. 6A and 6B), showing a predominance of hypo-DMRs. Furthermore, we show that hyper-DMRs are significantly depleted from CGI, but enriched in short interspersed elements (SINEs) and non-CGI regions. Strikingly, hypo-DMRs are specifically depleted from SINEs, with a concurrent enrichment in DNA transposons. Overall design: Examination of DNA methylome in zygotes from normal and HFD mice.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Wenjie Shu  

PROVIDER: GSE97109 | GEO | 2017-03-28

SECONDARY ACCESSION(S): PRJNA380689

REPOSITORIES: GEO

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Publications

Embryonic defects induced by maternal obesity in mice derive from Stella insufficiency in oocytes.

Han Longsen L   Ren Chao C   Li Ling L   Li Xiaoyan X   Ge Juan J   Wang Haichao H   Miao Yi-Liang YL   Guo Xuejiang X   Moley Kelle H KH   Shu Wenjie W   Wang Qiang Q  

Nature genetics 20180219 3


Maternal obesity can impair embryo development and offspring health, yet the mechanisms responsible remain poorly understood. In a high-fat diet (HFD)-based female mouse model of obesity, we identified a marked reduction of Stella (also known as DPPA3 or PGC7) protein in oocytes. Starting with this clue, we found that the establishment of pronuclear epigenetic asymmetry in zygotes from obese mice was severely disrupted, inducing the accumulation of maternal 5-hydroxymethylcytosine modifications  ...[more]

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