Genomics

Dataset Information

39

Immune repertoire after immunization as seen by next generation sequencing and proteomics


ABSTRACT: We study the repertoire of immunoglobulin sequences after immunization in a cohort of rats. Animals were immunized with dinitrophenol modified KLH or with recombinant human HuD. In the immune reprtoire, and also in the matching proteomics data, we observe that the animals produce a repertoire that has many shared motifs for those immunized with the same antigen. Cluster analysis allows the samples to be segregated according to the immunogen used. Overall design: 5 animals were immunized with DNP-KLH and 5 animals were immunized with human recombinant HuD. The immunoglobulin heavy chain variable domain was amplified from splenocyte cDNA, and sequenced with a 2x300bp paired end method

INSTRUMENT(S): Illumina MiSeq (Rattus norvegicus)

SUBMITTER: Martijn Maurice vanDuijn  

PROVIDER: GSE98855 | GEO | 2017-10-04

SECONDARY ACCESSION(S): PRJNA386462

REPOSITORIES: GEO

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Publications

Immune Repertoire after Immunization As Seen by Next-Generation Sequencing and Proteomics.

VanDuijn Martijn M MM   Dekker Lennard J LJ   van IJcken Wilfred F J WFJ   Sillevis Smitt Peter A E PAE   Luider Theo M TM  

Frontiers in immunology 20171016


The immune system produces a diverse repertoire of immunoglobulins in response to foreign antigens. During B-cell development, VDJ recombination and somatic mutations generate diversity, whereas selection processes remove it. Using both proteomic and NGS approaches, we characterized the immune repertoires in groups of rats after immunization with purified antigens. Proteomics and NGS data on the repertoire are in qualitative agreement, but did show quantitative differences that may relate to dif  ...[more]

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