Project description:This SuperSeries is composed of the following subset Series: GSE36242: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (RNA-Seq) GSE36243: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (Affymetrix) Refer to individual Series
Project description:Our study focuses on searching for novel genes involved in cholesterol metabolism through genome-wide screening, RNA-seq, proteome and lipidomic.This project maily focus on the proteomics of LDLR and HMGCR double knock out human HepG2 cells.
Project description:NGLY1 deficiency is a rare genetic disorder caused by mutations in the NGLY1 gene. This disorder presents a wide range of clinical symptoms, and its severity varies among affected individuals. Previous studies have focused on understanding the influence of NGLY1 on energy metabolism, revealing dysregulation in lipid metabolism following NGLY1 deletion. In this study, we investigated the consequences of the loss of NGLY1 on iron homeostasis using human hepatocellular carcinoma cells, HepG2. Comparative proteomics analysis revealed significant alterations in protein quantities in NGLY1-deficient HepG2 cells that suggested ferroptosis was prominently upregulated. Moreover, dysregulated iron uptake and increased reactive oxygen species (ROS) production were observed in the absence of NGLY1, indicating a novel perspective on the consequences of the loss of NGLY1. These findings provide important insights into the molecular pathways affected by NGLY1 deletion and may contribute to the development of potential therapeutic strategies for individuals with NGLY1 deficiency.
Project description:no27_iron_signaling - iron response - Effect of exceed of Iron - Time course experiment, star design and 3 biological repeats. Keywords: time course
