Project description:Wild type and atdamt1 seedlings were heat treated for 2.5 hours, control and treated seedlings were collected to extract total RNA for mRNA-seq. We then performed gene expression profiling analysis using data obtained from mRNA-seq.
Project description:Wild type and alkbh1a/1b seeds were stratified for two days, dry and stratified seeds were collected to extract total RNA for mRNA-seq. We then performed gene expression profiling analysis using data obtained from mRNA-seq.
Project description:Total extracts of four Collemataceae (C. auriforme, C. cristatum, C. fuscovirens and Leptogium lichenoides) collected in Austria, near Graz
Project description:The larvae were treated with 200 μM neomycin to deplete the hair cells in neuromast and ampullary organ. Two kinds of sensory epithelia at the ventral side of head were separated at 12 hour-post treatment (hpt) and 24 hpt, respectively. Mechano- and electro- sensory epithelia before neomycin treating were collected respectively as control (untreated).The epithelia near but without two kinds of sensors (EPs) were also collected. Total RNAs were extracted by TRIzol (Invitrogen) using standard protocols. RNA libraries were prepared by TruSeq RNA Sample Prep Kit (Illumina), following the manufacturer's instructions.
Project description:We sought to determine the transcriptomic impacts of artemisinin, Artemisia annua extract, and Artemisia afra extract on M. tuberculosis. Log phase cultures were treated with lethal doses for four hours or with inhibitory or sub-inhibitory doses for 24 hours. RNA was collected from untreated controls at the same timepoint.
Project description:Cxcl14+/- mice were mated with Cxcl14+/- mice, the embryos of pregnant females were collected on E13.5. After genotyping, the maternal part and fetal part of Cxcl14-/- and Cxcl14+/+ placentas were dissect out to extract total RNA respectively (n = 3). Total RNA was extracted using PureYield™ RNA Midiprep System (Promega).The maternal part of placenta that contained mesometrial lymphoid aggregate of pregnancy (MLAp) and decidua basalis (DB); the fetal part ofplacenta consisted of spongiotrophoblast and labyrinthin layer.Microarray hybridization was performed using a Mouse NimbleGen cDNA Microarray Kit (Roche), at CapitalBio Co., Ltd.(Beijing,China). Primary data were scaned using NimbleGen MS200 (Roche), and then extracted using NimbleScan system (Roche).
Project description:To investigate the molecular effect of Hypnea musciformis macroalgea extract on HepG2 and Ls174 cancer cell lines we isolated total RNA from cultured cells with and without the extract after 24 hr incubation We then performed gene expression profiling analysis performed in biological triplicates using data obtained from 3’ RNA-seq of 2 different cells with and without the extract at the IC50 concentration
Project description:Peripheral blood samples from children enrolled in the URECA cohort were collected at age 2. In short, at each URECA research center, within 16 hours of blood collection, mononuclear cells were separated and incubated (48 hours, 37C, 5% CO2) in the presence of German cockroach extract (10mg/mL; CR), dust mite extract (Dermatophagoides pteronyssinus, 10mg/mL; DM), tetanus toxoid (5mg/mL; TT), or medium alone (NS).