Project description:The microorganism was cultivated in M1 medium for 10 days, 28 centigrade degrees and 150 rpm followed by obtaining microbial extract with liquid-liquid extraction using ethyl acetate.
Project description:Mycobacterium tuberculosis H37Rv (Mtb) was grown in Sauton's medium with and without added zinc. Cultures were grown for 10 days and RNA was harvested from TRIzol extractions.
Project description:A natural product algal extract and its major component, cymopol, have demonstrated anti-oxidant and anti-inflammatory activity in vitro and in vivo. RNA transcript levels of Nqo1 and Hmox1 were discovered in multiple organs following oral gavage of 2 doses of the extract and cympopol for 3 consecutive days. The highest change in gene expression was noted in the digestive tract.
Project description:rs07-01_carnitine - treatment g-butyrobetaine wt col.0 - Effect of carnitine on the transcriptome of A. thaliana - Seedlings WT are growing in a independent way on media MS. After seven days of culture, seedling are harvested and a pull is formed from the three prelevements. Extractions of RNA will be realised on this pull. We proceed in a identical way for the test sample. Seedlings are cultivated on media MS containing butyrobétaïne 1 mM. After seven days, seedlings are harvested and extractions of RNA will be realised on this second pull. All this experiment is repeated one more time independently. Keywords: treated vs untreated comparison
Project description:To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene containing oleic acid analogue, as a bioorthogonal probe. We here show that this lipid can be readily taken up by dendritic cells without toxic side-effects, and that it can subsequently be visualised in live cells using an inverse electron-demand Diels-Alder (IEDDA) reaction with quenched tetrazine fluorophores. Furthermore, this reaction can be integrated into a multiplexed bioorthogonal reaction workflow by combining it with two sequential copper-catalysed Huisgen ligation reactions. This allows for the study of multiple biomolecules in the cell simultaneously by multimodal confocal imaging. Aside from lipid imaging, the uptake and protein modification of sterculic acid can be studied in live cells via chemical proteomics.
2023-04-28 | PXD033831 | Pride
Project description:Monitoring Multiple Myeloma in liquid biopsies