Project description:Human iPSCs and NSCs were engineered by AAVS1 and/or C13 safe-harbor TALENs which mediated targeted integration of various reporter genes at single or dual safe-harbor loci. Multiple clones of targeted human iPSCs were used to compare with parental untargeted NCRM5 iPSCs. Polyclonal targeted human NSCs were used to compare with their parental untargeted NCRM1NSCs or H9NSCs. Total RNA obtained from targeted human iPSCs or NSCs compared to untargeted control iPSCs or NSCs.
Project description:rs07-01_carnitine - treatment g-butyrobetaine wt col.0 - Effect of carnitine on the transcriptome of A. thaliana - Seedlings WT are growing in a independent way on media MS. After seven days of culture, seedling are harvested and a pull is formed from the three prelevements. Extractions of RNA will be realised on this pull. We proceed in a identical way for the test sample. Seedlings are cultivated on media MS containing butyrobétaïne 1 mM. After seven days, seedlings are harvested and extractions of RNA will be realised on this second pull. All this experiment is repeated one more time independently. Keywords: treated vs untreated comparison
Project description:This study aimed to characterize the non-targeted metabolite profile of Streptomyces sp. LZZY-S40 to explore its potential for producing bioactive secondary metabolites. The strain LZZY-S40 was cultivated on ISP 3 agar plates at 28°C for 7 days. Spores were harvested and inoculated into 250 mL Erlenmeyer flasks containing 50 mL of seed medium (pH 7.2–7.4) for 48 hours at 28°C with shaking at 250 rpm. The seed culture (8% v/v) was then transferred into fermentation medium and incubated under the same conditions for 7 days. After fermentation, the culture broth was centrifuged at 12,000 rpm for 10 minutes to separate the supernatant from the mycelial biomass. The supernatant was extracted three times with ethyl acetate, and the mycelial pellet was extracted with methanol. Organic extracts were concentrated under reduced pressure at temperatures below 40°C. The combined crude extracts were analyzed by untargeted liquid chromatography–mass spectrometry (LC–MS). Data were acquired in both positive and negative ionization modes across an m/z range of 50-1200, with chromatographic separation achieved using a gradient elution program at a flow rate of 400 μL/min and a column temperature of 40°C.
Project description:To identify genes regulated by AdpA in liquid culture, transcriptome profiles between delta adpA strains with his-adpA and control delta adpA strains were analyzed.
Project description:Microarray analysis of Aspergillus niger under conditions with differing combinations of carbon source, nitrogen source, nitrogen concentration, and culture pH Fermentor cultures were grown in minimal medium (MM) at a constant temperature of 30 ± 0.5 ºC and with differing combinations of carbon source (either 277.5 mM glucose or 333.0 mM xylose), nitrogen source (NH4Cl or NaNO3) and nitrogen concentration (4x: 282.4 mM; 8x: 564.8 mM), and pH (pH4 or pH5) of the medium (M. Braaksma, A.K. Smilde, M.J. van der Werf, P.J. Punt, submitted for publication). At different time points samples were collected, quenched immediately in methanol at -45 ºC and centrifuged at -20 ºC to remove supernatant. Part of the biomass was frozen into liquid nitrogen and stored at -80 ºC for microarray analysis. For each of the 16 culture conditions one sample was selected for microarray analysis; samples were collected either around the time point carbon source depleted or a considerable time (~24 h) after carbon souce depletion. In addition some technical duplicates were included.
Project description:ngs2014_39_leafnet2-cuc2-identification-Identification of CUC2 targets-10-days old 35S::CUC2-GR Arabidopsis transgenic line grown in liquid culture have been treated for 6 hours with DEXAMETHASONE 10µM or Mock.