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A Time-resolved Multi-omic Atlas of the Developing Mouse Stomach

ABSTRACT: We collected 3 replicates of whole stomach organs at 15 time points that covered embryonic days (E12.5, E13.5, E14.5, E15.5, E16.5, E17.5, E18.5), postnatal days (D1, D3, D5), and postnatal weeks (W1, W2, W3, W6, W8). The sampling time window covered from the earliest day that stomach sampling is anatomically feasible to the latest weeks when stomachs are close to mature. We applied a fast-seq proteomics workflow, a label-free quantitative proteomics approach in combination with small scale reverse phase pre-fractionation strategy.

ORGANISM(S): Mus Musculus

SUBMITTER: Jun Qin

PROVIDER: PXD010702 | iProX | Mon Aug 06 00:00:00 BST 2018

REPOSITORIES: iProX

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A time-resolved multi-omic atlas of the developing mouse stomach.

Li Xianju X Zhang Chunchao C Gong Tongqing T Ni Xiaotian X Li Jin'e J Zhan Dongdong D Liu Mingwei M Song Lei L Ding Chen C Xu Jianming J Zhen Bei B Wang Yi Y Qin Jun J

Nature communications 20181121 1

The mammalian stomach is structurally highly diverse and its organ functionality critically depends on a normal embryonic development. Although there have been several studies on the morphological changes during stomach development, a system-wide analysis of the underlying molecular changes is lacking. Here, we present a comprehensive, temporal proteome and transcriptome atlas of the mouse stomach at multiple developmental stages. Quantitative analysis of 12,108 gene products allows identifying ...[more]

PMID: 30464175

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