Discovery of Diurnal Variation of Plant Peptides Based on an Optimized Quantitative Peptidomics Approach
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ABSTRACT: Endogenous peptides mediate many biological events in multicellular organisms, but such function peptides have been rarely identified and characterized in plants. Here we developed an optimized quantitative peptidomics approach based on mass spectrometry, which is highly reproducible and efficient, as evidenced by the improved quality and quantity. Furthermore, this developed approach was used to characterize diurnal variation of maize peptides. Overall, 4676 peptides were identified and showed a time-dependent pattern. Of which, 14.16% (662) of endogenous peptides showed daily rhythms in abundance, largely in a phase-specific manner. The peak phases of rhythmic peptides were clustered around the time point 6 h after lights off, which was inconsistent with the peaks at dawn and dusk identified at the transcript level. Surprisingly, 40% of hub peptides identified by co-expressed analysis are diurnal rhythmic, highlighting the prominent roles of endogenous peptides in plant daily rhythm. Notably, further analysis showed that majority of the identified peptides in this study are initiated at non-AUG codons, indicating that non-AUG translation events are more widespread in plants than previously thought. Summarily, this study provides an optimized peptiodomics workflow for large-scale identification and quantification of endogenous peptide. In addition, it is the first report of plant peptides with diurnal variation, which indicating the potential function of plant endogenous peptides in circadian control.
ORGANISM(S): Zea Mays
SUBMITTER: Liuji Wu
PROVIDER: PXD019715 | iProX | Thu Jun 11 00:00:00 BST 2020
REPOSITORIES: iProX
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