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Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis

ABSTRACT: Toxoplasma gondii is an obligate intracellular parasite that can cause severe toxoplasmosis in humans and animals. Although T. gondii infection can disrupt the host's transcription, translation, metabolism and cause kidney damage, the mechanism remains largely unknown.By using a label-free proteomics quantitative method, we sequenced the urine proteome of female BALB/c mice at different phases of toxoplasmosis. A total of 1,802 proteins were obtained and proteome profiling of difference that easily distinguished between acutely infected groups and other groups was found. In this study, 169 and 47 differentially expressed proteins (DEPs) were found during the acute and chronic infection phases, respectively. Gene Ontology analysis showed that a large number of the these DEPs were associated with biological binding activity and single-organism process. KEGG pathway enrichment analysis showed that the majority of DEPs were involved in disease-related and metabolic pathways. Collectively, we have conducted in-depth exploration of mouse urine protein and revealed that urine protein variations were affected by the disease development process. This study provides new data on protein variations in urine of mice infected with T. gondii and can further understand the pathogenesis of mice. This research will help in the finding a key differential proteins, and provide effective data support for finding new treatment methods of toxoplasmosis.

ORGANISM(S): Mus Musculus Mus <mouse, Genus>

SUBMITTER: Chunxue Zhou

PROVIDER: PXD022458 | iProX | Tue Nov 10 00:00:00 GMT 2020

REPOSITORIES: iProX

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Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis.

Cui Lin-Lin LL Zhou Chun-Xue CX Han Bing B Wang Sha-Sha SS Li Si-Ying SY Xie Shi-Chen SC Zhou Dong-Hui DH

Parasites & vectors 20210420 1

<h4>Background</h4>Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis. Urine is an easily obtained clinical sample that has been widely applied for diagnostic purposes. However, changes in the urinary proteome during T. gondii infection have never been investigated.<h4>Methods</h4>Twenty four-hour urine samples were obtained from BALB/c mice with acute infection [11 days post infection (DPI)], mice with chronic infection (35 DPI) and healthy controls, and were anal ...[more]

PMID: 33879238

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Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.

Dataset Information

Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis

Publications

Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis.

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