Dataset Information

ITRAQ-based differential proteomic analysis in Sprague Dawley rats and Kunming mice brains chronically infected with type II Toxoplasma gondii strain (Prugniaud)

ABSTRACT: We artificially infected Sprague Dawley (SD) rats and Kunming (KM) mice with type II Toxoplasma gondii (T. gondii) strain (Prugniaud, Pru) to generate toxoplasmosis, which is a fatal disease mediated by T. gondii invasion in the central nervous system (CNS) with unknown mechanisms. We aimed to explore the mechanism of differential susceptibility of mice and rats to T. gondii infection. Therefore, we established a strategy of isobaric tags for relative and absolute quantitation (iTRAQ) to identify differentially expressed proteins (DEPs) of the rats’ and the mice’s brains compared to the healthy groups.

ORGANISM(S): Rattus Norvegicus Mus Musculus

SUBMITTER: Ziguo Yuan

PROVIDER: PXD011192 | iProX | Tue Sep 25 00:00:00 BST 2018

REPOSITORIES: iProX

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			Action	DRS
	20151008_mous&rat_1-1.raw	Raw
	20151008_mous&rat_1-10.raw	Raw
	20151008_mous&rat_1-11.raw	Raw
	20151008_mous&rat_1-12.raw	Raw
	20151008_mous&rat_1-2.raw	Raw

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Publications

iTRAQ-based differential proteomic analysis in Mongolian gerbil brains chronically infected with Toxoplasma gondii.

Lv Lin L Wang Yapei Y Feng Weili W Hernandez Jorge A JA Huang Wanyi W Zheng Yuxiang Y Zhou Xue X Lv Shumei S Chen Yajun Y Yuan Zi-Guo ZG

Journal of proteomics 20170318

The aim of our study was to detect differentially regulated proteins and specific signaling pathways in Mongolian gerbil brains during chronic Toxoplasma gondii (T.gondii) PRU strain infection. We use a iTRAQ-based strategy to detecte 4935 proteins, out of which 110 proteins were differentially expressed (>/=2.0-fold, p value <0.05) when the brain of gerbils infected with T.gondii was compared to control brain tissues. We confirmed the authenticity and the accuracy of iTRAQ results through quant ...[more]

PMID: 28323244

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