Proteomics

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Integrated proteomic and transcriptomic analyses reveal the roles of Brucella BI-1 in cell division and membrane homeostasis of Brucella suis S2

ABSTRACT: To explore the role of Brucella BI-1 in Brucella suis S2, we constructed the Brucella BI-1 deletion mutant strain and its complementary strain. We then determined the effect of Brucella BI-1 deletion on the physiological characteristics of Brucella suis S2 and revealed them via integrated transcriptomic and proteomic analyses. Brucella BI-1 deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting Brucella BI-1 led to defective growth, cell division, and viability in Brucella suis S2. In conclusion, our results revealed that Brucella BI-1 is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.

ORGANISM(S): Brucella Suis Bv. 1 Str. S2

SUBMITTER: Aihua Wang  

PROVIDER: PXD022537 | iProX | Fri Nov 13 00:00:00 GMT 2020

REPOSITORIES: iProX

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Integrated Proteomic and Transcriptomic Analyses Reveal the Roles of <i>Brucella</i> Homolog of BAX Inhibitor 1 in Cell Division and Membrane Homeostasis of <i>Brucella suis</i> S2.

Zhang Guangdong G   Zhong Fangli F   Chen Lei L   Qin Peipei P   Li Junmei J   Zhi Feijie F   Tian Lulu L   Zhou Dong D   Lin Pengfei P   Chen Huatao H   Tang Keqiong K   Liu Wei W   Jin Yaping Y   Wang Aihua A  

Frontiers in microbiology 20210128

BAX inhibitor 1 (BI-1) is an evolutionarily conserved transmembrane protein first identified in a screening process for human proteins that suppress BAX-induced apoptosis in yeast cells. Eukaryotic BI-1 is a cytoprotective protein that suppresses cell death induced by multiple stimuli in eukaryotes. <i>Brucella</i>, the causative agent of brucellosis that threatens public health and animal husbandry, contains a conserved gene that encodes BI-1-like protein. To explore the role of the <i>Brucella  ...[more]

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