Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomics reveals Steap3-Gm2a axis controlling lysosomal escape of Listeria monocytogenes in murine macrophages

ABSTRACT: As a pathogenic microorganism, Listeria monocytogenes is widely used in the study of bacterial pathogenic mechanism and host defense mechanism. The lysosomal escape of Listeria monocytogenes is essential for its replication in the cytoplasm. Here, we report that Listeria monocytogenes infection can cause the expression level of Six-transmembrane epithelial antigen of the prostate 3 (Steap3) to decrease in macrophages. Functional experiments show that Steap3 can inhibit lysosomal escape of Listeria monocytogenes. Then, the comprehensive proteomic analysis reveals that the deletion of Steap3 affects the lysosomal signaling pathway of macrophages. Among these proteins affected by Steap3, we discover that Ganglioside GM2 activator (Gm2a) may be a potential target gene of Steap3. In summary, we find that Steap3-Gm2a axis can inhibits lysosomal escape of Listeria monocytogenes and serve the potential molecular drug targets for antibacterial treatment.

ORGANISM(S): Mus Musculus

SUBMITTER: Jiangbei Yuan

PROVIDER: PXD025697 | iProX | Thu Apr 29 00:00:00 BST 2021

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Intracellular transcription by multiple Listeria monocytogenes strains

Project description:These studies were designed to examine the transcription of Listeria monocytogenes strains 10403S and LO28 during intracellular replication in mammalian macrophages. Duplicate WT Listeria monocytogenes (strains 10403S and LO28) were used to infect mouse bone marrow-derived macrophages (BMMs). Bacterial RNA was harvested at 4 hours post-infection.

2011-12-02 | E-GEOD-34083 | biostudies-arrayexpress

Gene expression profile in peritoneal macrophage infected with Listeria monocytogenes

Project description:Several Toll-like receptors are activated by Listeria monocytogenes infection, resulting in the activation of MyD88 dependent signaling pathway. However, the negative role of MyD88 in gene expresson is unclear. To address this, we performed microarray analysis of mRNAs from WT or MyD88-/- peritoneal macrophages infected with Listeria monocytogenes.

2014-06-29 | GSE52115 | GEO

Intracellular transcription by multiple Listeria monocytogenes strains

Project description:These studies were designed to examine the transcription of Listeria monocytogenes strains 10403S and LO28 during intracellular replication in mammalian macrophages.

2011-12-02 | GSE34083 | GEO

Immunoproteome profiling of Listeria monocytogenes under mild acid and salt stress conditions

Project description:Listeria monocytogenes is the ubiquitous food-borne pathogen which causes listeriosis, a disease with a high mortality rate, mostly transmitted through contaminated ready-to-eat foods (EFSA, 2018). To better understand the systemic response of such microorganism exposed at three environmental factors (T, pH and NaCl), the proteome of a L. monocytogenes strain, which was isolated from a meat product (Coppa di testa) linked to a listeriosis outbreak occurred in Marche region (Italy) in 2016, was investigated in order to identify differences in its protein patterns.

2022-08-12 | PXD033519 | Pride

Listeria infection in hepatocytes

Project description:Listeria monocytogenes causes severe foodborne illness in pregnant women and immunocompromised individuals. After the intestinal phase of infection, the liver plays a central role in the clearance of this pathogen through its important functions in immunity. However, recent evidence suggests that subpopulations of L. monocytogenes may escape eradication after prolonged infection of hepatocytes, by entering a persistence phase in vacuoles. Here, we examine whether this long-term infection alters hepatocyte defense pathways, which may be instrumental for bacterial persistence. We first established models of Listeria infection in human hepatocyte cell lines HepG2 and Huh7 and in primary mouse hepatocytes (PMH). In these cells, Listeria efficiently enters the persistence stage after a 3-day infection, while inducing a type I (PMH) or type I/III (HepG2) or no (Huh7) interferon response. RNA-seq analysis identified a common signature of long-term Listeria infection on the hepatocyte transcriptome, characterized by overexpression of a set of genes involved in antiviral immunity and under-expression of many acute phase protein (APP) genes, particularly involved in the complement and coagulation systems. The decrease in APP transcript amounts correlated with lower protein abundance in the secretome of infected cells, as shown by proteomics, and also occurred in the presence of APP inducers (IL-6 or IL-1b). The results also suggest that long-term Listeria infection affects lipid metabolism pathways. Collectively, these results reveal that long-term infection with L. monocytogenes profoundly deregulates the innate immune functions of hepatocytes, which could generate an environment favorable to the establishment of persistent infection.

2021-11-02 | PXD027154 | Pride

The molecular mechanism of AP-2 complex degradation of LLO secreted by Listeria in the cytoplasm

Project description:Listeria monocytogenes is a common clinical pathogen primarily transmitted among humans and animals through contaminated food. Currently, the increasing prevalence of antibiotic resistance due to the misuse of antibiotics has become a significant problem, leaving both clinical medicine and agriculture with a lack of effective treatments for Listeria infections. Listeriolysin O (LLO), a virulence factor secreted by Listeria monocytogenes, is a key factor in its pathogenicity. Strains of Listeria monocytogenes lacking the LLO gene are non-pathogenic to humans and animals. Therefore, studying the molecular mechanisms of LLO degradation is of great practical significance for treating clinical infections caused by antibiotic-resistant Listeria. Additionally, it will provide a theoretical basis for developing new antimicrobial drugs. Since Listeria monocytogenes secretes LLO throughout its entire life cycle within the host cell, understanding the fate of LLO at different stages of infection is crucial for elucidating the pathogenic mechanisms of Listeria monocytogenes. It is already known that LLO secreted by Listeria within the phagosome can be degraded via the lysosomal pathway. However, the fate of LLO secreted by Listeria in the cytoplasm remains poorly understood. Based on our previous experimental data and relevant literature, we propose a novel hypothesis that the AP-2 complex targets and degrades LLO secreted by Listeria in the cytoplasm.

2025-01-25 | PXD060183 |

Comparative transcriptomics of pathogenic and non-pathogenic Listeria species

Project description:Listeria monocytogenes is a human, food-borne pathogen. Genomic comparisons between L. monocytogenes and Listeria innocua, a closely related non-pathogenic species, were pivotal in the identification of protein coding genes essential for virulence. However, no comprehensive comparison has focused on the non-coding genome. We used strand-specific cDNA sequencing to produce genome-wide transcription start site (TSS) maps for both organisms, and developed a publicly available integrative browser to visualize and analyze both transcriptomes in different growth conditions and genetic backgrounds. Our data revealed conservation across most transcripts, but significant divergence between the species in a subset of non-coding RNAs. In L. monocytogenes we identified 113 sRNAs and 70 asRNAs, significantly increasing the repertoire of non coding RNAs in this species. Remarkably, we identified a class of long antisense transcripts (lasRNAs) that overlap one gene while also serving as the 5M-bM-^@M-^Y UTR of the adjacent divergent gene. Experimental evidence suggests that lasRNAs transcription inhibits expression of one operon while activating the expression of another. Such lasRNA/operon structure, termed "excludon", might represent a novel form of regulation in bacteria. Construction of consensus TSS-maps in Listeria monocytogenes and Listeria innocua by applying 5'-end sequencing on samples in different conditions and genetic backgrounds.

2012-05-01 | E-GEOD-36060 | biostudies-arrayexpress

Transcriptome analysis of Listeria monocytogenes infected bone marrow derived macrophages with or without DEAD-box helicase DDX3X

Project description:Bone marrow derived macrophages were infected with Listeria monocytogenes for 4 hours. We investigated differently expressed genes in the absence of DDX3X upon infection and also in steady state conditions.

2018-10-17 | GSE86591 | GEO

Expression data from macrophages of E-selectin+/+ and E-selectin-/- mouse infected with Listeria monocytogenes in vivo on day3

Project description:We used microarrays to detail the global gene expression in peritoneal macrophages (PM) from E-selectin+/+ and E-selectin-/- mouse infected with Listeria Monocytogenes in vivo on day3

2018-01-25 | GSE90808 | GEO

Expression data from CCR2+ and CCR2- splenic marophages from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

Project description:We used microarrays to detail the global gene expression in CCR2+ and CCR2- spenic macrophages (SM) sorted from C57BL6 mouse infected with Listeria Monocytogenes in vivo on day3

2019-01-01 | GSE97790 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data