CheA pupylation sites identified by SPIDER assay

ABSTRACT: We used CheAs and biotin-CheZ to validate the efficiency of SPIDER capturing protein-protein interaction. Pupylation sites on WT CheAs after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e., GGE, that covalently binds to adjacent lysine by pupylation. ）

ORGANISM(S): Escherichia Coli

SUBMITTER: Shengce Tao  

PROVIDER: PXD026509 | iProX | Fri Jun 04 00:00:00 GMT+01:00 2021

REPOSITORIES: iProX