Pupylation sites on protein RutR
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ABSTRACT: We used RutR and specific biotin-DNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on RutR after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e., GGE, that covalently binds to adjacent lysine by pupylation. )
ORGANISM(S): Escherichia Coli
SUBMITTER: Shengce Tao
PROVIDER: PXD026516 | iProX | Fri Jun 04 00:00:00 BST 2021
REPOSITORIES: iProX
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