Pupylation sites on protein Sox2

ABSTRACT: As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e., GGE, that covalently binds to adjacent lysine by pupylation. ）

ORGANISM(S): Homo Sapiens

SUBMITTER: Shengce Tao  

PROVIDER: PXD026517 | iProX | Fri Jun 04 00:00:00 BST 2021

REPOSITORIES: iProX