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Genetically encoding histone mark for mapping the chromatin interactomes via living cell proximity labeling

ABSTRACT: We developed a method using site-specific genetic code expansion strategy in conjunction with APEX2-mediated proximity labeling to decipher interactors of hPTMs in living cells via quantitative proteomics. The method allows site-specific incorporation of a PTM-modified amino acid into histones that fused to APEX2, facilitating the labeling of the PTM-modified histones to binding proteins in vivo. Thus, this system will be a promising alternative strategy for interpreting the interactomes of hPTMs in cellular contexts.

ORGANISM(S): Homo Sapiens

SUBMITTER: Kai Zhang

PROVIDER: PXD031007 | iProX | Fri Jan 14 00:00:00 GMT 2022

REPOSITORIES: iProX

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