Genetically encoding histone mark for mapping the chromatin interactomes via living cell proximity labeling
Ontology highlight
ABSTRACT: We developed a method using site-specific genetic code expansion strategy in conjunction with APEX2-mediated proximity labeling to decipher interactors of hPTMs in living cells via quantitative proteomics. The method allows site-specific incorporation of a PTM-modified amino acid into histones that fused to APEX2, facilitating the labeling of the PTM-modified histones to binding proteins in vivo. Thus, this system will be a promising alternative strategy for interpreting the interactomes of hPTMs in cellular contexts.
ORGANISM(S): Homo Sapiens
SUBMITTER:
Kai Zhang
PROVIDER: PXD031007 | iProX | Fri Jan 14 00:00:00 GMT 2022
REPOSITORIES: iProX
ACCESS DATA