Proteomics of AH sample from Diabetic Macular Edema
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Xiaorong Li
PROVIDER: PXD033404 | iProX | Sun Apr 24 00:00:00 BST 2022
REPOSITORIES: iProX
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Project description:To investigate the changes in aqueous humor (AH) protein profiles before and after intravitreal aflibercept (IVA) treatment in patients with proliferative diabetic retinopathy (PDR) patients. 5 PDR patients provided 10 samples of AH before and after IVA treatment (pre-group vs post-group). Proteins were identified using liquid chromatography-tandem mass spectrometry. Then, bioinformatics was employed to investigate the functional significance of differentially expressed proteins (DEPs) and hub proteins.
2024-05-21 | PXD039210 | Pride
Project description:Diabetic retinopathy (DR) is a leading cause of irreversible vision impairment and blindness. To explore the dynamics of aqueous humor (AH) protein profiles during four stages from non-diabetic individuals to proliferative diabetic retinopathy patients, especially to improve our understanding of pathophysiological mechanisms of proliferative diabetic retinopathy (PDR).
2025-05-21 | PXD046630 |
Project description:Diabetic retinopathy (DR) is a leading cause of irreversible vision impairment and blindness. To explore the dynamics of aqueous humor (AH) protein profiles during four stages from non-diabetic individuals to proliferative diabetic retinopathy patients, especially to improve our understanding of pathophysiological mechanisms of proliferative diabetic retinopathy (PDR).
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Project description:To better understand the molecular changes in the aqueous humor (AH) content with glaucoma, we analyzed the microRNA (miRNA) profiles of AH samples from patients with Primary Open Angle Glaucoma (POAG) and Exfoliation Glaucoma (XFG) compared to non-glaucoma controls.
2018-02-08 | GSE105269 | GEO
Project description:PURPOSE: To investigate the circulatory microRNA (miRNA) profiles of aqueous, vitreous, and plasma in order to identify biomarkers in aqueous humor or plasma that are reflecting changes in vitreous of patients with diabetes. METHODS: Aqueous, vitreous and plasma samples were collected from a total of 27 patients - 11 controls (macular pucker or macular hole patients) and 16 patients with diabetes mellitus (DM) undergoing vitreoretinal surgery: DM-Type I with proliferative diabetic retinopathy (PDR) (DMI-PDR), DM Type II with PDR (DMII-PDR) and DM Type II with nonproliferative DR (DMII-NPDR). MiRNAs were isolated using Qiagen microRNeasy kit, quantified on BioAnalyzer, labeled with FlashTag kit, and profiled on Affymetrix GeneChip miRNA 3.0 microarrays. Data analysis was done using Expression Console (EC), Transcriptome Analysis Console (TAC), and Ingenuity Pathway Analysis (IPA) software. RESULTS: Our comparison of circulatory miRNA population of aqueous and vitreous humor and plasma showed that out of total of 847 human miRNA probes on the Affymetrix GeneChip miRNA 3.0 we found common miRNAs for both aqueous and vitreous samples, as well as larger number of unique miRNA, dependent on the DM type and presence of retinopathy. Most of the dysregulated miRNAs in aqueous and vitreous of DM patients were upregulated, while in plasma, most of the DM-specific miRNAs were downregulated. Dysregulation of miRNAs in aqueous generally do not appear to be a good representative of the miRNA abundance in vitreous, or plasma, although we did identify a few candidates for common biomarkers: let-7b, miR-320b, miR-762 and miR-4488. Additionally, each of the DR subtypes showed a set of miRNA that is uniquely dysregulated in each fluid, for example in aqueous samples for DMII-NPDR it was miR-455-3p, for DMII-PDR was miR-296, and for DMI-PDR it was miR-3202. Pathway analysis identified TGF-beta and VEGF pathways as the common targets for miRNAs dysregulated in DR aqueous and vitreous. CONCLUSIONS: The comparative profiling of circulatory miRNAs in aqueous, vitreous, and plasma showed that a small number of circulatory miRNAs displayed differential presence in controls vs. diabetic retinopathy. A pattern is emerging of sets of miRNA that are common or uniquely dysregulated in the blood plasma or ocular fluids of DR subtypes, offering promise for the use of ocular fluids and plasma for identifying diagnostic and therapeutic targets.
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Project description:Diabetic retinopathy (DR) is a leading cause of blindness in adults under 40 in the developed world, with a significant proportion progressing to vision-threatening stages like proliferative diabetic retinopathy (PDR) and neovascular glaucoma (NVG). This study aims to explore the molecular mechanisms underlying the progression from non-proliferative DR (NPDR) to PDR and NVG, focusing on identifying potential biomarkers and therapeutic targets. We utilized discovery-based proteomics, specifically label free quantification (LFQ), to analyze aqueous humor (AH) proteins from patients with NPDR, PDR, and NVG from the AH samples obtained during cataract surgery or anterior chamber paracentesis. We employed triple quadrupole (QQQ)-MS for targeted protein quantification to validate marker proteins for each disease state. Notably,subsequent analyses revealed specific proteins associated with each stage of DR. Gene ontology analysis and functional annotation unveiled significant processes in DR such as immune response and lipid metabolic process. Validation of potential biomarkers revealed proteins showing significant concentration changes, with several candidates demonstrating diagnostic potential through ROC curve analysis. These findings provide insights into the biomarkers and pathogenesis of these severe neovascular complications. The identification of specific proteins associated with the progression of DR to more advanced stages offer a promising avenue for early diagnosis and targeted therapy. The study underscores the potential of AH proteomics in understanding complex diseases like DR and its complications.
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Project description:The focus of our research centers on exploring the proteome changes and corresponding signaling pathways involved in the development of diabetic cataract, age-related cataract and post-vitrectomy cataract, investigating aqueous humor, anterior capsule and lens human samples. Outstanding proteomic profiles of the three sample types have been generated using DIA proteomics. We have observed substantial differences in the regulation of several pathways between our groups and samples types, and we have particularly highlighted the involvement of non-canonical Wnt receptor signaling pathway, glycosaminoglycan and glycosphingolipid pathways, and oxidation reduction pathway in the formation of different cataract forms.
2025-05-06 | PXD045557 | Pride
Project description:The focus of our research centers on exploring the proteome changes and corresponding signaling pathways involved in the development of diabetic cataract, age-related cataract and post-vitrectomy cataract, investigating aqueous humor, anterior capsule and lens human samples. Outstanding proteomic profiles of the three sample types have been generated using DIA proteomics. We have observed substantial differences in the regulation of several pathways between our groups and samples types, and we have particularly highlighted the involvement of non-canonical Wnt receptor signaling pathway, glycosaminoglycan and glycosphingolipid pathways, and oxidation reduction pathway in the formation of different cataract forms.
2025-05-06 | PXD045554 | Pride
Project description:The focus of our research centers on exploring the proteome changes and corresponding signaling pathways involved in the development of diabetic cataract, age-related cataract and post-vitrectomy cataract, investigating aqueous humor, anterior capsule and lens human samples. Outstanding proteomic profiles of the three sample types have been generated using DIA proteomics. We have observed substantial differences in the regulation of several pathways between our groups and samples types, and we have particularly highlighted the involvement of non-canonical Wnt receptor signaling pathway, glycosaminoglycan and glycosphingolipid pathways, and oxidation reduction pathway in the formation of different cataract forms.
2025-05-06 | PXD045547 | Pride