TMT-Based Quantitative Proteomic Analysis of Intestinal Or-ganoids Infected by Listeria monocytogenes with Different Virulence

ABSTRACT: L. monocytogenes consisting of 13 serotypes, is an opportunistic food-borne pathogen that causes different host reactions to depend on its serotypes. In this study, highly toxic L. monocytogenes 10403s resulted in more severe infections and lower survival rates. Additionally, to investigate the remodeling of host proteome by strains exhibiting differential toxicity, the cellular protein re-sponses of intestinal organoids were analyzed using tandem mass tags (TMT) labeling and high-performance liquid chromatography-mass spectrometry. The virulent strain 10403s caused 102 upregulated and 52 downregulated proteins, while the low virulent strain M7 caused 188 upregulated and 25 downregulated proteins. Based on the analysis of Gene Ontology (GO) and KEGG databases, the expression of differential proteins in organoids infected by L. monocytogenes 10403s (virulent strain) or M7 (low virulent strain), was involved in regulating essential processes such as biological metabolism, energy metabolism, and immune system process. The results showed that the immune system process, as primary host defense response caused by L. mono-cytogenes, had five pathways including ECM-receptor interaction, complement and coagulation cascade, HIF-1, Ferroptosis, and NOD-like receptor signaling pathways. These results revealed that L. monocytogenes strains with different toxicity induced similar biological functions and immune responses while having different regulations on differential proteins in the pathway.

ORGANISM(S): Mus Musculus

SUBMITTER: Keping Ye  

PROVIDER: PXD034151 | iProX | Fri May 27 00:00:00 BST 2022

REPOSITORIES: iProX