Dataset Information

Immune Responses to multi-frequency of 1.5GHz and 4.3GHz microwave exposure in rats

ABSTRACT: In this study, we exposed the rats to 1.5 GHz (L10), 4.3 GHz (C10) or multi-frequency (LC10) microwave at an av-erage power density of 10 mW/cm2. Both single and multi-frequency microwave induced slight pathological changes in thymus and spleen. And, the white blood cells (WBCs) and lymphocytes in peripheral blood were decreased at 6 h and 7 d after exposure, suggesting immune suppressive responses were induced. Among lymphocytes, the B lymphocytes were increased while the T lymphocytes were decreased at 7 d after exposure in C10 and LC10 groups, but not in L10 group. Moreover, multi-frequency microwave regulated the B and T lymphocytes stronger than C band microwave. The results of proteomics showed that both single and mul-ti-frequency microwave regulated numerous genes associated with immune regulation and cel-lular metabolism in peripheral blood and spleen. However, multi-frequency microwave altered the expression of much more proteins. Moreover, multi-frequency microwave down-regulated T lymphocytes development, differentiation and activation associated proteins, while up-regulated B lymphocytes activation related proteins. In conclusion, multi-frequency mi-crowave of 1.5 GHz and 4.3 GHz produced immune suppressive responses via regulating im-mune regulation and cellular metabolism associated proteins. Our findings provided meaningful information for exploring potential mechanisms underlying multi-frequency induced immune suppression.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Ruiyun Peng

PROVIDER: PXD034706 | iProX | Mon Jun 20 00:00:00 BST 2022

REPOSITORIES: iProX

ACCESS DATA

Publications

Immune Responses to Multi-Frequencies of 1.5 GHz and 4.3 GHz Microwave Exposure in Rats: Transcriptomic and Proteomic Analysis.

Zhao Li L Yao Chuanfu C Wang Hui H Dong Ji J Zhang Jing J Xu Xinping X Wang Haoyu H Yao Binwei B Ren Ke K Sun Liu L Peng Ruiyun R

International journal of molecular sciences 20220622 13

With the rapidly increasing application of microwave technologies, the anxiety and speculation about microwave induced potential health hazards has been attracting more and more attention. In our daily life, people are exposed to complex environments with multi-frequency microwaves, especially L band and C band microwaves, which are commonly used in communications. In this study, we exposed rats to 1.5 GHz (L10), 4.3 GHz (C10) or multi-frequency (LC10) microwaves at an average power density of 1 ...[more]

PMID: 35805954

Similar Datasets

Project description:Granulocyte-colony stimulating factor (G-CSF) is used to boost granulocyte counts in immunocompromised patients, but its effects on the immune system may be counter productive. We tested the hypothesis that G-CSF mobilized peripheral blood stem cell (PBSC) products are immunologically down regulated based on gene microarray analysis. Ten peripheral blood samples from normal donors for allogeneic PBSC transplantation were obtained before and after administration of G-CSF and tested on Affymetrix Human U133 Plus 2.0 GeneChipÂ® microarrays. Significant changes in gene expression after G-CSF mobilization were reported by controlling the false discovery rate at 5%. Immune-related genes were isolated from the data set and categorized according to probe set annotations and a thorough, independent literature search. We found that G-CSF up-regulated inflammatory and neutrophil activation pathway gene expression; however, adaptive immune-related gene expression, such as antigen presentation, co-stimulation, T cell activation and cytolytic effector pathways, were generally down-regulated. Thus, despite significant increases in stem cells, lymphocytes and antigen presenting cells, G-CSF mobilized PBSC allografts exhibit a suppressive adaptive immune-related gene expression profile. Our data provides an explanation for the potentially immunosuppressive effects observed after G-CSF administration. Experiment Overall Design: This study was approved by the University of Florida Institutional Review Board. Five healthy donors (A-E) for allogeneic PBSC transplantation consented and were mobilized with rHu G-CSF at 10Âµ/kg/day for five days. Approximately 4mL of venous blood was collected before (Pre-G-CSF) and after (Post-G-CSF) mobilization. Whole blood leukocyte RNA was purified from each sample and used to generate cRNAs which were subsequently hybridized onto Affymetrix Human U133 Plus 2.0 GeneChipÂ® microarrays. Representative genes were successfully validated with quantitative RT-PCR.

Dataset Information

Immune Responses to multi-frequency of 1.5GHz and 4.3GHz microwave exposure in rats

Publications

Immune Responses to Multi-Frequencies of 1.5 GHz and 4.3 GHz Microwave Exposure in Rats: Transcriptomic and Proteomic Analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure