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ITRAQ-based proteomic study investigate the effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins

ABSTRACT: Purpose: This study aimed to investigate the variation of MFGM proteins in colostrum of Hu sheep under different dietary crude protein levels. Experimental design: Collecting the colostrum from Hu sheep fed three different levels of dietary crude protein levels. Plasma membrane proteins were enriched, digested, labeled using an iTRAQ kit, and identified by LC-MS. Differentially expressed proteins were analyzed using bioinformatics technology. Results: Pair-wise comparisons among three treatments identified 286 differentially expressed MFGM proteins. Bioinformatics analysis suggested that the three most abundant differentially expressed MFGM proteins were signaling, disulfide-bonded, and secreted proteins.

ORGANISM(S): Ovis Aries

SUBMITTER: Xiangpeng Yue

PROVIDER: PXD037465 | iProX | Mon Oct 17 00:00:00 BST 2022

REPOSITORIES: iProX

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Effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins and the performance of Hu ewes and their offspring.

Wang Zhongyu Z Zhang Nana N Li Fadi F Yue Xiangpeng X

Frontiers in veterinary science 20221130

Dietary proteins play important roles in the growth and reproduction of sheep, and the ewe's demand for proteins increases dramatically during late pregnancy. This research aimed to investigate the effect of dietary crude protein (CP) levels during late pregnancy on colostrum fat globule membrane (MFGM) protein and the growth performance of Hu sheep and their offspring, and provide a reference for the protein intake of ewes during late pregnancy. A total of 108 multiparous Hu sheep (45.6 ± 1.18 ...[more]

PMID: 36532352

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