ITRAQ-based proteomic study investigate the effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins

ABSTRACT: Purpose: This study aimed to investigate the variation of MFGM proteins in colostrum of Hu sheep under different dietary crude protein levels. Experimental design: Collecting the colostrum from Hu sheep fed three different levels of dietary crude protein levels. Plasma membrane proteins were enriched, digested, labeled using an iTRAQ kit, and identified by LC-MS. Differentially expressed proteins were analyzed using bioinformatics technology. Results: Pair-wise comparisons among three treatments identified 286 differentially expressed MFGM proteins. Bioinformatics analysis suggested that the three most abundant differentially expressed MFGM proteins were signaling, disulfide-bonded, and secreted proteins.

ORGANISM(S): Ovis Aries

SUBMITTER: Xiangpeng Yue  

PROVIDER: PXD037465 | iProX | Mon Oct 17 00:00:00 BST 2022

REPOSITORIES: iProX